RNAprep pure FFPE Kit

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For purification of RNA from formalin-fixed, paraffin-embedded tissues

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• StorageDNase I, Buffer RDD, RNase-Free ddH₂O (Tubular) should be stored at 2-8℃.Other reagents could be store at room temperature (15-25℃)
• DescriptionThe RNAprep Pure FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions can remove formaldehyde modifications of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase I and Buffer RDD for optimized removal of genomic DNA contamination. The purified RNA can be used in various of downstream applications such as RT-PCR.
• Required ReagentsXylene, ethanol
• Features■ Silica membrane based technology to ensure high-purity of RNA.■ Fast and simple process compared with conventional methods.■ Suitable for downstream RT-PCR or RT-qPCR applications.
• ApplicationsThis kit is suitable for the purification of total RNA from formalin-fixed, paraffin-embedded tissue sections (FFPE).

Publication

.. 104 / Publication

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• IGF-1R mediates crosstalk between nasopharyngeal carcinoma cells and osteoclasts and promotes tumor bone metastasisJournal of Experimental & Clinical Cancer Research : CR2024-02-12 Read Article.. paraffin sections was extracted using the RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer’s instructions for
• Comprehensive analysis of miRNA-mRNA regulatory pairs associated with colorectal cancer and the role in tumor immunity.BMC genomics2023-12-04 Read ArticleTotal RNA was extracted from FFPE tissues using RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer??s instructions.
• Comprehensive analysis of miRNA-mRNA regulatory pairs associated with colorectal cancer and the role in tumor immunityBMC Genomics2023-11-30 Read ArticleTotal RNA was extracted from FFPE tissues using RNAprep Pure FFPE Kit (TIANGEN) according to the manufacturer??s instructions.
• Construct dysregulated miRNA-mRNA interaction networks to conjecture possible pathogenesis for Stomach adenocarcinomasCancer Biomarkers2023-11-23 Read ArticleAbstract: BACKGROUND: Post-transcriptional regulation of mRNA induced by microRNA is known crucial in tumor occurrence; progression; and metastasis.. This study aims at identifying significant miRNA-mRNA axes for stomach adenocarcinomas (STAD).. METHOD: RNA expression profiles were collected from The Cancer Genome Atlas (TCGA) and GEO database for screening differently expressed RNAs and miRNAs (DE-miRNAs/DE-mRNAs).
• Long noncoding RNA XLOC_006786 inhibits the proliferation, invasion and metastasis of osteosarcoma cells through NOTCH3 signaling pathway by targeting miR-491-5p.Human cell2023-11-01 Read ArticleAbstract: Recent research has indicated that Long noncoding RNAs (LncRNAs) are crucial in many disorders; especially tumors.. However; the exact role of LncRNA XLOC_006786 (LncRNA-SPIDR-2:1) in malignancies; especially in human osteosarcoma; is unclear.. The results of RT??qPCR; western blotting; CCK-8 assays; and Transwell assays showed that LncRNA XLOC_006786 inhibited osteosarcoma cell proliferation; invasion; and migration; indicating that it may be a tumor suppressor gene in osteosarcoma.

RNAprep pure FFPE Kit

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