GDP316-TIANamp Micro DNA Kit

TIANamp Micro DNA Kit
For isolation of genomic DNA from small volumes of blood
dried blood spots plasma and serum mouthwash hair follicles tissues
microdissected tissues

TIANamp Micro DNA Kit
(Spin Column)
Cat. no. GDP316

Storage
TIANamp Micro DNA Kit can be stored at room temperature (15-30°C)
for up to 15 months without showing any reduction in performance and
quality. If any precipitate forms in the buffers, it should be dissolved by
warming the buffers at 37°C for 10 min before use. Stock solution of
Carrier RNA should be stored at -30~-15°C.

Introduction
TIANamp Micro DNA Kit is based on silica membrane technology and
provides special buffer system. Genomic DNA binds to the silica-membrane
in the presence of high salt, while the contaminants pass through the
column. After the membrane is thoroughly washed to remove any
remaining contaminants, the pure DNA is eluted from the membrane with
low salt buffer.
The kit is suitable for a wide range of sample materials such as small
volumes of blood, dried blood spots, serum/plasma, tiny amount of
tissues, mouthwash, hair follicles. Purified genomic DNA can directly serve
as templates for PCR, restriction enzyme digestion, hybridization, etc.

Preparation of Carrier RNA Stock Solution
At the first use of Carrier RNA, add 310 μl RNase-Free ddH2O to the tube containing 310 μg lyophilized Carrier RNA to obtain a solution of 1 μg/μl. Dissolve the Carrier RNA thoroughly, divide it into conveniently sizedaliquots, and store at -20°C. Do not freeze-thaw the aliquots of Carrier RNA more than 3 times.

Protocol:
<1> Genomic DNA from Small Volumes of Blood
Ensure that ethanol (96-100%) has been added to Buffer GD and PW
according to the instructions at the first use.
<2> Isolation of Genomic DNA from Dried Blood Spots
Ensure that ethanol (96-100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.
<3> Isolation of Circulating Nucleic Acid/cell free Nucleic Acid from
Plasma/Serum
Ensure that ethanol (96-100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.
<4> Isolation of genomic DNA from mouthwash
Ensure that ethanol (96-100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.
<5> Isolation of Genomic DNA from hair follicles
Ensure that ethanol (96-100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.
Please prepare 1 M DTT solution before use.
<6> Isolation of Genomic DNA from tiny amount of tissues
Ensure that ethanol (96-100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.
<7> Isolation of Genomic DNA from Microdissected Tissues including
Formalin Fixed Microdissected Tissues
Ensure that ethanol (96–100%) has been added to Buffer GD and Buffer
PW according to the instructions at the first use.

GDP316-TIANamp Micro DNA Kit

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