GDP605-DE-TGuide Smart Universal DNA Kit

For purification genomic DNA from
blood, dried blood spot, bacteria, cells, saliva, oral
swabs, animal tissues, etc.

TECHNICAL MANUAL
Cat. no. GDP605-DE

Note: To use the TGuide Smart Universal DNA Kit, you must have the
TGuide Smart Universal DNA(program no. DP605-01/02) installed on the
TGuide S16/S32 pro Nucleic Acid Extractor.

Table Contents

Kit Contents
Universal DNA reagent composition
Storage condition
Materials to Be Supplied by the User
Product
Features
Notes
Operational steps
I. Preparation of universal DNA reagent composition
II. Sample pre-processing
III. Operation steps of TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products

TGuide Smart Universal DNA Kit
Cat. no. GDP605-DE

Materials to Be Supplied by the User
Isopropanol, 1M DTT, Lysozyme solution ,1M NaOH

1. Isopropanol should be prepared to extract any sample’s genomic DNA with this kit.
   Please read the relevant operational steps carefully and add isopropanol into 1st
   well of the single sample reagent cartridge in the specified order and volume.
2. 1M DTT should be self-provided for extracting genomic DNA from samples of hair
   and feather with hair follicles;
3. Lysozyme solution (50 mg/ml) (TIANGEN, Cat. no.: GRT401) and reaction buffer
   for preparing lysozyme (20 mM Tris, pH 8.0; 2 mM Na2-EDTA; 1.2% TritonX-100)
   should be self-provided for extracting bacterial genomic DNA.
4. 1M NaOH should be self-provided for extracting bacterial genomic DNA from
   sputum samples.

Product
The kit adopts magnetic beads and a unique buffer system, to isolate and purify
genomic DNA with high quality from blood, dried blood spot, bacteria, cells, saliva,
oral swabs, animal tissues and other samples. The uniquely embedded magnetic
beads have a strong affinity for nucleic acid under certain conditions. When the
conditions are changed, the magnetic beads can release the absorbed nucleic
acid to rapidly separate and purify it. The whole process is safe and convenient,
and the extracted genomic DNA has large fragments and high purity and is stable
and reliable in quality. It can perfectly fit with TGuide S16 Nucleic Acid Extractor of
TIANGEN for automated extraction.
DNA purified by this kit is suitable for downstream experiments including enzymatic
digestion, PCR, qPCR, library construction, Southern Blot, chip analysis and highthroughput sequencing.

Features

• Simple and fast:Ultra-pure total DNA can be obtained by running TGuide S16 within
  1 hr.
• Safe and non-toxic: No toxic reagents such as phenol/chloroform.
• Strong commonality: It can be used to extract genomic DNA from blood, tissues,
  swabs, bacteria, cells and other samples.
• High purity: The DNA obtained has high purity and can be directly used for chip
  detection, high-throughput sequencing and other experiments.

Notes

1. The sample should avoid repeated freezing and thawing, otherwise the DNA
   fragments extracted will be small and the amount of extraction will be reduced.
2. If there is precipitation in the Buffer GHA, it can be re-dissolved in 37°C water bath
   and used after mixing.
3. The pre-processing methods of different samples can be different. Please read the
   manual carefully before the experiment and prepare the reagent by users.
4. If you need a TGrinder H24R tissue homogenizer for electric homogenization of
   tissue, users need to purchase and ask TIANGEN for the grinding method.
5. After sample pre-processing, take an appropriate amount of the sample and add it
   to the 1st well of the single sample reagent cartridge.
6. There are two automated extraction programs in the operational steps of this kit,
   Pause program DP605-01 and Non-stop program DP605-02. Wherein, DP605-01
   is recommended to use in nucleic acid extraction from blood samples, and the
   instrument will automatically pause after the end of step 6. At this moment, users
   need to add 350 µl isopropanol in 1st well of the single sample reagent cartridge.
   Besides, if the sample is cells and users have a high demand for the purity of cell
   genomic DNA, it is also recommended to use the program DP605-01. Non-stop
   program DP605-02 is recommended to use in the nucleic acid extraction from
   other samples. Before the single sample reagent cartridge operation, users need
   to add 350 µl isopropanol in the 1st well , and then run the DP605-02 program.
7. The elution buffer is not prefilled, users need to add the elution buffer to the 5th
   well of the single sample reagent cartridge before performing on the instrument.
   The recommended range of elution buffer is 60~100 µl. The smaller the elution
   volume, the higher the nucleic acid concentration, but the lower the total yield
   may be. On the contrary, the larger the elution volume, the lower the nucleic acid
   concentration, and the higher the total yield may be. You can adjust it in the range
   of 60~100 µl as needed.

Operational steps

I. Preparation of universal DNA reagent composition

1. Take out a prefilled single sample reagent cartridge and invert it to re-suspend the
   magnetic beads; Gently shake to concentrate the reagent and magnetic beads to
   the bottom of the cartridge. Before use, remove the sealing film carefully to avoid
   liquid spatter or spills.
2. Add the Buffer TB of appropriate volume (60~100 µl) into the 5th well of the single
   sample reagent cartridge.
3. Please read the operation manual carefully, and pay attention to the pause step of
   DP605-01 in the extraction process. If users use the non-stop program DP605-02,
   please add 350 µl isopropanol into the 1st well before operation.

II. Sample pre-processing

A. Blood sample (anticoagulant)

B. Dried blood spot sample

III. Operation steps of TGuide S16 Nucleic Acid Extractor

1. Place the cartridge on the reagent tank bracket.
2. Place the reagent tank bracket on the base in the TGuide S16 Nucleic Acid
   Extractor. Insert the Tip Combs into the slots to ensure that they are well
   connected and firmed

Appendix
1.Program
The automated extraction program of Universal DNA kit is shown in the following table.
Table 1-1
DP605-01:Automated Extraction Program of Universal DNA (Blood Samples) by
TGuide S16 Nucleic Acid Extractor.

2. Related Products
   Instrument and Accessories

GDP605-DE-TGuide Smart Universal DNA Kit

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