morgan@go2biotech.comGDP604-DE-TGuide Smart Viral DNARNA Kit
TGuide Smart Viral DNA/RNA Kit
For purification viral DNA/RNA from blood, tissue, serum, plasma, body fluid, swab,
tissue sputum, etc.
TECHNICAL MANUAL
Cat. no. GDP604-DE
Note: To use the TGuide Smart Viral DNA/RNA Kit, you must have the
TGuide Smart Viral DNA/RNA(program no. DP604-01) installed on the
TGuide S16/S32 pro Nucleic Acid Extractor.
Table Contents
Table Contents
Kit Contents
Virus DNA/RNA reagent composition
Storage condition
Product
Features
Notes
Operational steps
1.Preparation of virus DNA/RNA reagent
2.Sample pre-processing
3.Operation steps of TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products
TGuide Smart Viral DNA/RNA Kit
Cat. no. GDP604-DE
Storage condition
The kit can be stored in dry conditions at room temperature (15~30°C) for 12months.
Product
The kit adopts magnetic beads and a unique buffer system, to isolate and purify
viral DNA/RNA with high quality from blood, tissue, serum, plasma, lymph, cell-free
body fluid, cell culture supernatant, urine, or various viral preservation solutions. The
uniquely embedded magnetic beads have a strong affinity for nucleic acid under
certain conditions. When the conditions are changed, the magnetic beads can
release the absorbed nucleic acid to rapidly separate and purify it. The extracted viral
DNA/RNA has high yield, high purity, stable and reliable quality. It can perfectly fit
with TGuide S16 Nucleic Acid Extractor of TIANGEN for automated extraction.
Nucleic acid purified by this kit is suitable for downstream experiments including RTPCR, PCR, and qPCR.
Features
- Simple and fast:High-quality viral DNA/RNA can be obtained by running TGuide
S16 within half an hour. - Flexible throughput:It is a perfect fit for TGuide S16 Nucleic Acid Extractor to
extract 1~8 samples. - Wide use: It is applicable to various cell-free body fluid samples, blood and tissue
suspensions
Notes
- The sample should be avoided repeated freeze-thaw, otherwise it will lead to
reduced nucleic acid extraction. The sample can be extracted immediately or stored
at 4°C for less than 24 hr. For long-term storage, it can be stored at -20°C or -80°C. - When extracting viral DNA/RNA by the kit, special attention should be paid to
prevent nucleic acid degradation. The utensils, sample injectors, etc. used should
be dedicated, and disposable consumables such as centrifuge tubes and pipette
tips should go through autoclaved sterilization. Operators should wear powderfree
gloves, masks, etc. - There may be magnetic beads residual in the 5th well at elution step, so try to
avoid touching magnetic beads during final sample transfer. If magnetic particles
are present in user’s storage tube, centrifuge at 10,000 × g for 2 minutes, and
transfer the supernatant to a clean tube (not provided). - The samples and reagent materials used should be disposed properly, and the
operating table should be cleaned and disinfected thoroughly. - It is necessary to take an appropriate amount of the processed sample and add it
to 1st well of the single sample reagent cartridge. - The elution buffer (RNase-Free ddH2
O) is not prefilled, so you need to add the
elution buffer (RNase-Free ddH2
O) to the 5th well of the single sample reagent
cartridge before performing on the instrument. The recommended range of elution
buffer is 60~100 µl. The smaller the elution volume, the higher the nucleic acid
concentration, but the lower the total yield may be. On the contrary, the larger the
elution volume, the lower the nucleic acid concentration, and the higher the total
yield may be. You can adjust it in the range of 60~100 µl as needed.
Operational steps
- Preparation of virus DNA/RNA reagent
1.1 Take out a prefilled single sample cartridge and invert it to re-suspend the
magnetic beads; Gently shake to concentrate the reagent and magnetic beads
to the bottom of the cartridge. Before use, remove the sealing film carefully to
avoid liquid spatter or spills.
1.2 Add an appropriate volume (60~100 µl) of elution buffer (RNase Free ddH2
O,
included in the kit) to 5th well of the single sample reagent cartridge. - Sample pre-processing
2.1 Take out the blood, serum, plasma, urine, pleural effusion and ascites, alveolar
lavage fluid, cerebrospinal fluid, saliva and other samples and balance them to
room temperature before loading to the cartridge, proceed to 3.1.
2.2 Swab samples
If it is a dry swab sample, add a particular volume of normal saline (preferably
immersing the swab), vortex it for 15~30 sec to mix well, and proceed to 3.1. If
the sample contains a swab and preservation solution, after vortex and mixing
well, and proceed to 3.1.
2.3 Tissue samples
Take the tissue blocks, add an appropriate amount of PBS buffer or normal
saline, homogenize the tissue, centrifuge it at 12,000 rpm for 2 min, and
proceed to 3.1.
2.4 Sputum samples
If the sputum is thick, add twice the volume of the normal saline for diluting,
and then add 20 µl Proteinase K and mix well, settle for 5~10 min to liquefy
the sample (Proteinase K is not needed in the next step), and proceed to 3.1. - Operation steps of TGuide S16 Nucleic Acid Extractor
3.1 Add 200 µl supernatant of the sample (the sample should be balanced to room
temperature) and 20 µl of Proteinase K into the 1st well of the single sample
reagent cartridge. And put the cartridge on the reagent tank bracket.
3.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
Acid Extractor. Insert the Tip Combs into the slots to ensure they are well
connected and firmed.
3.3 If you use the TGuide S16 Nucleic Acid Extractor, select the corresponding
program DP604-01 file on the touch screen, click the icon in the lower right
corner of the screen and click the “RUN” button at the bottom of the screen to
start the experiment.
3.4 At the end of the automated extraction process, take the DNA/RNA out of the
5th well of the single sample reagent cartridge and store it under appropriate
conditions. Single sample reagent cartridge and tip comb are for single use
only
Appendix
1.Program
The automated extraction process of virus DNA/RNA is shown in the following table:
- Related Products
Instrument and Accessories
GDP604-DE-TGuide Smart Viral DNARNA Kit
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