morgan@go2biotech.comGDP642-DE-TGuide Smart DNA Purification Kit
For purification of total RNA from plant tissues.
TECHNICAL MANUAL
Cat. no. GDP662-DE
Note: To use the TGuide Smart Magnetic Plant RNA Kit, you must have the
TGuide Smart Magnetic Plant RNA (program no. DP662) installed on the
TGuide S16/S32 pro Nucleic Acid Extractor.
Table Contents
Kit Contents
Plant RNA reagent composition
Storage condition
Product
Features
Notes
Before the first use
Operational steps
1.Prefilled single sample cartridge
2.Attention to sample pre-processing
3.Start the TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products
TGuide Smart Magnetic Plant RNA Kit
Cat. no. GDP662-DE
Storage condition
All components of the kit can be stored in dry conditions at room temperature
(15~30°C) for 12 months. RNase-Free DNase I, Buffer RDD and RNase-Free
ddH2O (in tubes) can be stored at 2~8°C for 15 months.
Product
This product adopts magnetic beads and a unique buffer system to isolate and purify
high quality total RNA from plant tissues. The uniquely embedded magnetic beads
have a strong affinity for nucleic acid under certain conditions. When the conditions
are changed, the magnetic beads can release the absorbed nucleic acid to rapidly
separate and purify it.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through
absorption, transfer and release of magnetic beads by the special magnetic bar,
magnetic beads and nucleic acid can be transferred to improve the degree of
automation. The whole process is safe and convenient. The total RNA extracted has
good purity and high yield.
Total RNA purified by this kit can be used for RT-PCR, qPCR, chip analysis, Northern
Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis,
molecular cloning and other downstream experiments.
Features
- Simple and fast:Ultra-pure total RNA can be obtained by running TGuide S16 for 58
minutes. - Flexible throughput: It can perfectly fit with TIANGEN TGuide S16 Nucleic Acid
Extractor to extract 1-8 samples. - Safe and non-toxic: No toxic reagents such as phenol/chloroform High purity: The
RNA obtained has high purity and can be directly used in downstream experiments
such as chip detection and high-throughput sequencing.
Notes
- This product is suitable for TGuide S16 Nucleic Acid Extractor.
- Pay attention to the pause step(~30mins after running program DP662). User needs
to add 700 μl Buffer RD into the 3rd well manually before the program can resume. - Pay attention to the optimal storage and pre-processing conditions of samples to
avoid degradation of extracted RNA. - If the TGrinder H24R tissue homogenizer is needed for electric homogenization of
plant tissues, you could buy it and ask TIANGEN for a grinding scheme (OSE-TH-02).
Before the first use
- Prepare DNase I stock solution : Dissolve the lyophilized DNase I (1500 units) in
550 μl of the RNase-free ddH2O. Do not remove the rubber top of the vial to prevent
loss of DNase I powder. Use a syringe and a needle to inject the RNaseFree ddH2O
into the vial. Mix gently by inverting. Do not vortex. Divide the solution into singleuse aliquots, and store at -30~-15°C for up to 9 months. Thawed aliquots can be stored at 2-8°C for up to 6 weeks. Do not refreeze the aliquots after thawing. - Add absolute ethanol into Buffer RD according to the volume stated on the label of
the bottle.
Operational steps
- Prefilled single sample cartridge
1.1 Take out a prefilled single sample cartridge and invert to re-suspend the
magnetic beads; Gently shake to concentrate the reagent and magnetic beads
to the bottom of the cartridge. Before use, remove sealing film carefully to
avoid liquid spatter or spills.
1.2 Add proper volume (60~100 μl) of RNase-Free ddH2
O to the 5th well of the
cartridge.
- Sample pre-processing:
Note: For samples with rich polysaccharide content, such as pear leaves and
strawberry leaves, it is not recommended to use this kit for extraction.
Take fresh or -80°C frozen plant tissue and fully grind with liquid nitrogen into
powder. Weigh 50~100 mg and add to a 1.5 ml centrifuge tube containing 700
μl Buffer SL (or use TIANGEN’s TGrinder H24R tissue homogenizer for electric
homogenization of plant tissues, and complete 1~24 plant samples for 1 min,
without liquid nitrogen), to which, add 20 μl Proteinase K, for immediate vortex
mixing. Let it stand it at room temperature for 5 min, centrifuge at 12000 rpm for 5
min, and carefully take the supernatant.
Note: For samples with polysaccharides and polyphenols, it is recommended
to increase the amount of buffer SL to 1 ml and take 600 μl supernatant after
centrifugation for subsequent operations. - Start the TGuide S16 Nucleic Acid Extractor
3.1 Add 600 μl supernatant obtained above to the 1st well of the cartridge. Add 10 μl
pre-prepared DNase I stock solution with 70 μl Buffer RDD to the 3rd well. Place
the cartridge on the reagent tank bracket of TGuide S16 Nucleic Acid Extractor.
3.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
Acid Extractor. Insert the Tip Combs into the slots to ensure that they are well
connected and firmed.
3.3 If you use the TGuide S16 Nucleic Acid Extractor, select the corresponding
program DP662 file on the touch screen, click the icon in the lower right
corner of the screen, or click the “RUN” button at the bottom of the screen to
start the experiment.
3.4 At the pause step, add 700 μl Buffer RD into the 3rd well manually and then
click “Confirm”.
3.5 At the end of the automated extraction process, take the RNA out of the 5th
well of the cartridge and store it under appropriate conditions. If there are
subsequent experiments, it can be stored at 4°C for no more than 4 hours.
Single sample reagent cartridge and tip comb are for single use only.
Appendix
- Program
The extraction process of S16 provided for DP662 is shown in the following table
GDP642-DE-TGuide Smart DNA Purification Kit
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
