GDP607-DE-TGuide Smart Magnetic Plant DNA Kit

GDP607-DE-TGuide Smart Magnetic Plant DNA Kit

For purification of genomic DNA from various plant tissues.

TECHNICAL MANUAL
Cat. no. GDP607-DE

Note: To use the TGuide Smart Magnetic Plant DNA Kit, you must have the
TGuide Smart Magnetic Plant DNA (program no. DP607) installed on the
TGuide S16/S32 pro Nucleic Acid Extractor.

Table Contents

Kit Contents
Plant DNA reagent composition
Storage condition
Product
Features
Notes
Operational steps
1.Prefilled single sample cartridge
2.Sample processing
3.Operation steps of TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products

TGuide Smart Magnetic Plant DNA Kit
Cat. no. GDP607-DE

Storage condition
All components of the kit can be stored in dry conditions at room temperature
(15~30°C) for 12 months. If the solution precipitates, it can be preheated in a
water bath at 37°C for 10 min before use to dissolve the precipitation, without
affecting the effect.

Product
This product adopts magnetic beads and a unique buffer system to isolate and
purify high-quality genomic DNA from various plant tissues. The uniquely embedded
magnetic beads have a strong affinity for nucleic acid under certain conditions.
When the conditions are changed, the magnetic beads can release the absorbed
nucleic acid to rapidly separate and purify the nucleic acid.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through
absorption, transfer and release of magnetic beads by the special magnetic bar,
magnetic beads and nucleic acid can be transferred to improve the degree of
automation. The whole process is safe and convenient, and the extracted genomic
DNA fragments are large, with high purity and reliable quality.
The DNA purified by this kit is suitable for a range of common downstream
applications including digestion, PCR, library construction, Southern hybridization,
and other experiments

Features

Simple and fast: Ultra-pure genomic DNA can be obtained by running TGuide S16
for 60 minutes.
Widely applicable: It is suitable for a variety of plant tissues, especially
polysaccharide or polyphenol-rich plants.
Safe and non-toxic:No toxic organic reagents such as phenol/chloroform.
High purity:The obtained DNA has high purity and can be directly used for chip
detection, high-throughput sequencing and other experiments

Notes

Repeated freezing and thawing samples should be avoided, otherwise the extracted
DNA fragments will be small and the total yield will decrease.
If there is precipitation in the buffer GPS, it can be dissolved in a 37°C water bath
and used after shaking well.

Operational steps

Prefilled single sample cartridge
1.1 Take out a prefilled single sample cartridge and invert it to re-suspend the
magnetic beads; Gently shake to concentrate the reagent and magnetic beads
to the bottom of the cartridge. Before use, remove the sealing film carefully to
avoid the liquid spatter or spills.
1.2 Add proper volume (60~100 μl) of elution buffer TB to the 5th well of the
cartridge.

Sample processing
2.1 Take approximately 100 mg fresh tissue or 30 mg dry tissue to grind with liquid
nitrogen or a homogenizer.
2.2 Transfer the ground powders rapidly into centrifuge tubes pre-loaded with 400
μl Buffer GPS and 20 μl Proteinase K. After mixing them upside down quickly,
place samples in a water bath at 65°C for 15 min. During the incubation, invert
the centrifuge tubes to mix samples several times.
2.3 Add 4 μl RNase A (100 mg/ mL) and mix it thoroughly, and let it stand at room
temperature for 5 min.
2.4 Add 100 μl Buffer GPA and fully mix it, followed by ice bath incubation for 5
min and centrifugation at 12,000 rpm for 5 min.
Note:If the solution will be sticky after Step 2, please increase the use of
Buffer GPS and Buffer GPA proportionally, e.g., use 600 μl Buffer GPS and 150 μl
Buffer GPA.

Operation steps of TGuide S16 Nucleic Acid Extractor
3.1 Add 450 μl supernatant obtained after processing the above sample to the
1st well of the prefilled single sample cartridge, and place the cartridge on the
reagent tank bracket of TGuide S16 Nucleic Acid Extractor.
Note:Do not touch bottom impurities when sucking supernatant, with the
volume of transferred supernatant below 500 μl.
3.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
Acid Extractor. Insert the Tip Combs into the slots to ensure that they are well
connected and firmed.

3.3 If you use the TGuide S16 Nucleic Acid Extractor, select the corresponding
program DP607 file on the touch screen, click the icon in the lower right
corner of the screen, or click the “RUN” button at the bottom of the screen to
start the experiment.
3.4 At the end of the automated extraction process, take the DNA out from the 5th
well of the cartridge and store it under appropriate conditions. Single sample
reagent cartridge and tip comb are for single use only.