GDP602-DE-TGuide Smart Magnetic Tissue DNA Kit

For genomic DNA purification from tissue and cells.

TECHNICAL MANUAL
Cat. no. GDP602-DE

Note: To use the TGuide Smart Magnetic Tissue DNA Kit, you must have
the TGuide Smart Magnetic Tissue DNA (program no. DP602) installed on
the TGuide S16/S32 pro Nucleic Acid Extractor.

Table Contents

Kit Contents
Tissue DNA reagent composition
Storage condition
Product
Features
Notes
Operational steps
1.Prefilled single sample cartridge
2.Sample processing
3.Operation steps of TGuide S16 Nucleic Acid Extractor
Appendix
1.Program
2.Related Products

TGuide Smart Magnetic Tissue DNA Kit
Cat. no. GDP602-DE

Storage condition
The kit can be stored under dry conditions at room temperature (15~30°C)
for 12 months. If the solution precipitates, it can be preheated in a water bath
at 37°C for 10 min to dissolve the precipitation, without affecting the effect.

Product
This kit adopts magnetic beads and a unique buffer system to separate and purify
high-quality genomic DNA from various animal tissues and cells . The uniquely
embedded magnetic beads have a strong affinity for nucleic acid under certain
conditions. When the conditions are changed, the magnetic beads can release the
absorbed nucleic acid to rapidly separate and purify the nucleic acid.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through
absorption, transfer and release of magnetic beads by the special magnetic bar,
magnetic beads and nucleic acid can be transferred to improve the degree of
automation. The whole process is safe and convenient, and the extracted genomic
DNA fragments are large, with high purity and reliable quality.
The DNA purified by this kit is suitable for a range of common downstream
applications including digestion, PCR, library construction, Southern hybridization,
and other experiments.

Features

• Simple and fast: Ultra-pure genomic DNA can be obtained by running TGuide S16
  for 50 minutes.
• Wide use: It is applicable to all kinds of animal tissues.
• Ultra-pure: The obtained DNA has high purity and can be directly used in PCR,
  digestion, hybridization and other molecular biological experiments.

Note

1. Repeated freezing and thawing samples should be avoided, otherwise the
   extracted DNA fragments will be small and the total yield will decrease.
2. If there is precipitation in the Buffer GHA, it can be dissolved in a 37°C water bath
   and used after shaking well.

Operational steps

1. Prefilled single sample cartridge
   1.1 Take out a prefilled single sample cartridge and invert it to re-suspend the
   magnetic beads; Gently shake to concentrate the reagent and magnetic beads
   to the bottom of the cartridge. Before use, remove sealing film carefully to
   avoid liquid spatter or spills.
   1.2 Add proper volume (60~100 μl) of buffer TB to the 5th well of the cartridge.

2. Sample processing
   2.1 Tissue
   Take 10~50 mg of animal tissue, use a liquid nitrogen or high-throughput tissue
   grinding homogenizer (TGrinder H24R tissue homogenizer, self-prepared,
   TIANGEN: OSE-TH-02) to adequately grind the tissue, or cut tissue into small
   pieces as much as possible, then add 400 μl Buffer GHA and 20 μl Proteinase K.
   Proceed to 3.1.
   1) Samples with tissue blocks visible to naked eyes are recommended to be
   digested at 65°C for 30 min until completely digested;
   2) Samples with sufficient homogenization do not need the above digestion
   process.
   3) The rat tail samples should be digested overnight at 56°C.
   Notes: After sample digestion, if there are tissue fragments, it is recommended
   to centrifuge at 12,000 rpm for 1 min to remove residual impurities. If RNA
   needs to be removed, add 4 μl RNase A (100 mg/ml) to the supernatant
   solution after centrifugation, and place it at room temperature for 10 min.

2.2 Cell
1) Processing methods of different cell samples:
(1) Suspension cell: Determine the number of cells collected (the collected
number should not be more than 1×10
7
) and centrifugate at 300 × g for
5 min. Then collect cells into a centrifuge tube and carefully remove all
supernatant of the culture medium. Wash the cells with PBS solution, and
then suck out the PBS solution as much as possible. Add 100 μl PBS to the
cells, and completely re-suspend the cells.
(2) Adherent cell: Determine the number of cells and remove the culture
medium. Wash cells with the PBS solution, and suck out the PBS solution.
Then add the PBS solution containing 0.10~0.25% trypsin to cells for
digestion. When the cells are released from the wall of the vessel, add a
medium containing serum to inactivate the trypsin. Transfer the cell solution
to an RNase-free centrifuge tube and centrifuge it at 300 × g for 5 min.
Collect cell pellets and carefully remove all supernatant. Add 100 μl PBS to
the cells, and completely re-suspend the cells.
Note: When collecting cells, it is important to remove all cell culture medium;
otherwise, it will lead to incomplete digestion.
2) Add 200 μl Buffer GHA and 20 μl Proteinase K to the collected cell pellets,
and completely re-suspend the cells.
Note. To remove RNA, add 4 μl RNaseA (100 mg/mL), shake it for 15 sec, and
incubate at room temperature for 5 min.
3) Proceed to 3.1.

3. Operation steps of TGuide S16 Nucleic Acid Extractor
   3.1 Add 300 μl solution after processing the above sample in the 1st well of the
   cartridge. Put cartridges on the reagent tank bracket of TGuide S16 Nucleic
   Acid Extractor.
   3.2 Place the reagent tank bracket on the plate base in the TGuide S16 Nucleic
   Acid Extractor. Insert the Tip Combs into slots to ensure that they are well
   connected and firmed

3.3 If you use the TGuide S16 Nucleic Acid Extractor, select the corresponding
program DP602 file on the touch screen, click the icon in the lower right
corner of the screen, or click the “RUN” button at the bottom of the screen to
start the experiment.
3.4 At the end of the automated extraction process, take the DNA out of the 5th
well of the cartridge and store it under appropriate conditions. Single sample
reagent cartridge and tip comb are for single use only.

Appendix

1. Program
   The extraction process of S16 provided for DP602 is shown in the following table

GDP602-DE-TGuide Smart Magnetic Tissue DNA Kit

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