KT221-Golden Easy PCR System-191021

Golden Easy PCR System
Cat. no. 4993004

Storage:
Store at -30~-15°C for up to two years and avoid repeated freezing and thawing. For regular use, please
store at 2-8°C for up to three months.

Introduction
Golden Easy PCR Kit is a double component Easy PCR System which contains Golden DNA Polymerase and 2×Reaction Mix. It is characterized by rapidity, simplicity, high sensitivity, strong specificity, good stability andminimizing human errors. It is suitable for routine PCR reaction, amplification and large-scale gene detection of complex templates such as high GC content (>60%) and
of secondary structure..

Product Components
1) 2× Reaction Mix: 500 µM dNTP each
20 mM Tris-HCl (pH8.3)
100 mM KCl
3 mM MgCl2
Stabilizer and enhancer
2) Golden DNA Polymerase: 2.5 U/µl

Quality control
No exogenous nuclease activity was detected. Effectively amplify single copy genes in human genome. After one week storage at room temperature (15-30°C), there was no obvious change in activity.

Description
This product is designed to allow the user for quick and easy preparation of reaction mixture, which can avoid contaminating during the PCR operating process. For PCR reaction set-up, the user just need to pipette an aliquot part of 2× Reaction Mix and Golden DNA Polymerase,
dilute the Reaction Mix to 1× by adding templates, primers and water up to the reaction volume. After PCR amplification, PCR products of Loading dye Mix can be loaded directly without the additional loading buffer. 2×Reaction Mix and Golden DNA Polymerase are separated in this kit to master the enzyme dosage flexibly according to specific experiment. If the enzyme is added to the 2×
Reaction Mix, it can be stably stored.

Application

• Gene detection:there is almost no difference between
  different batches of this product, especially suitable
  for large-scale gene detection, semi-quantitative PCR
  experiment and trace DNA detection.
• Complex template amplification (secondary structure,
  GC rich and repeat sequence):PCR products with A
  can be cloned directly with T/A vector after purification

Example
Note: The following example is only for reference,user must set up optimal reaction system according to
different reaction conditions such as different templates or primers etc.
1.For 25 µl PCR reaction system: 1 kb fragment of human genomic DNA was amplified by the Golden Easy PCR System (If use different reaction system, please proportionally increase or decrease the amount of reaction components referring to this system).

PCR reaction cycle set-up:

3. Result detection: After the reaction, 5 µl of the reaction
   product was detected by agarose gel electrophoresis

KT221-Golden Easy PCR System-191021

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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