DP344-Magnetic Blood Spots DNA Kit

Magnetic Blood Spots DNA Kit
Magnetic bead method kit capable of purifying DNA in highthroughput rapid from dry blood spot

Magnetic Blood Spots DNA Kit
Cat.no. 4992720/4992721

Storage
This kit can be stored at room temperature (15-30°C) for 15 months. If
any precipitate forms in the buffers, it should be dissolved by warming the
buffers at 37°C before use.

Introduction
The kit adopts magnetic beads with unique separation function and a
unique buffer system to separate and purify high-quality genomic DNA
from dried blood spots. The unique embedded magnetic beads have
strong affinity for nucleic acid under certain conditions, and when the
conditions change, the magnetic beads will release adsorbed nucleic acid,
thus achieving the purpose of fast separation and purification of nucleic
acid. The whole process is safe and convenient. The extracted genomic
DNA fragments are large, with high purity, stable and reliable quality. The
method is especially suitable for automatic extraction of high-throughput
workstations.

Features

• Easy and fast:Ultrapure genomic DNA can be obtained within 1 h.
• High Throughput: It can be adapted to the automated instruments of
  pipetting-based method and magnetic rod method to carry out high
  throughput extraction experiments.
• Safe and non-toxic:No reagent such as phenol/chloroform is needed.
• High purity: The obtained DNA has high purity and can be directly used
  in chip detection, high-throughput sequencing and other experiments.

Important Notes Before Using

1. This product is suitable for both manual extraction or automatic
   instrument integration.
2. Self-prepared reagents: Isopropanol, ethanol.
3. If there are precipitates in Buffer GAS and Buffer GHC, they can be
   redissolved in a 37°C water bath and used after thoroughly mixing by
   shaking.

Determination of DNA Concentration and Purity
The size of the purified genomic DNA fragment is related to factors such
as sample storage time and shearing force during the operation. The
concentration and purity of the DNA fragments can be detected by agarose
gel electrophoresis and UV spectrophotometer. DNA should have a significant absorption peak at OD260, with OD260 value of1 equivalent to about 50 μg/ml double stranded DNA and 40 μg/ml single
stranded DNA.
The ratio of OD260/OD 280should be 1.7-1.9. If using deionized water instead
of elution buffer, the ratio will be low, because the pH value and the
presence of ions will affect the light absorption value, but it does not mean
the purity is low.

DP344-Magnetic Blood Spots DNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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