morgan@go2biotech.comDP115-TIANprep Rapid N96 Plasmid Kit
TIANprep Rapid N96 Plasmid Kit
TIANprep Rapid N96 Plasmid Kit
Cat. no. 4992868/4992869
(N96 Plate)
Storage
TIANprep Rapid N96 Plasmid Kit can be stored at room temperature (15-30°C) for up to 15 months without showing any reduction in performance
and quality. If any precipitate forms in the buffers, it should be dissolved
by warming the buffers at 37°C before use. RNase A (10 mg/ml) can
be stored for 15 months at room temperature (15-30°C). After adding
RNase A, Buffer P1 is should be stored at 2-8°C and is stable for 6 months.
Introduction
TIANprep Rapid N96 rapid plasmid DNA extraction kit is suitable for rapid
preparation of high-throughput plasmids, and may complete plasmid DNA
extraction of 96 samples within 2-3 hours. This kit adopts the special N96
Filtration Plate (H) and Buffer III, which may quickly and efficiently extract
high purity plasmid DNA.
The plasmid DNA purified by the kit is suitable for large-scale sequencing
and conventional molecular biological operations, such as enzyme
digestion, library screening, ligation, transformation, etc.
Important Notes Please read the notes before using this kit.
- Culture method of 96 well plate bacteria: Add 1.0-1.3 ml of culture
medium containing corresponding antibiotics to each well of 96 deepwell plates, then pick a single colony into each well to culture. Seal the
plate with permeation to prevent contamination, and culture at 220-280 rpm 37°C. - RNase A shall be added to the Buffer P1 before use (please prepare in
batches, add 1.25 ml RNase A per 125 ml P1, which may be used for
4-plate extraction reaction), mix well, and store at 2-8°C. - Before use, check whether Buffer P2 and Buffer III are turbid. If there is
turbid phenomenon, incubate in a 37°C water bath for a few minutes to
restore clarification. Cover Buffer P2 and Buffer III immediately after use. - All centrifugation steps shall be conducted at room temperature.
- The yield of extracted plasmid is related to factors such as bacterial
culture concentration and plasmid copy number.
Operation instructions of TIANRed
TIANRed is a color indicator to indicate the correctness of the whole
operation, which does not affect any downstream experiments and is
harmless to human body. TIANRed is an optional reagent, and customers
may choose whether to add it or not according to their needs.
Usage method: Before use, add according to TIANRed:P1=1:200, and
mix it upside down until it is completely uniform. The Buffer P1 added
with TIANRed is red when in use; After Buffer P2 is added, the red color
completely turns purple, indicating full lysis; after Buffer III is added
again, the homogeneous state is yellow, indicating that neutralization and
renaturation are sufficient.
DP115-TIANprep Rapid N96 Plasmid Kit
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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