OSR-M610-B-TGuide Total RNA Whole Blood Kit

TGuide Total RNA Whole Blood Kit

TGuide Total RNA Whole Blood Kit
Cat. No. OSR-M610-B

Storage Conditions:
RNase-free DNase I, Buffer RDD and RNase-free ddH2
O (tube) are stored
at 2-8°C for 12 months. Buffer RBB added with β-mercaptoethanol can be
stored for one month at 2-8°C, and other reagents can be stored at room
temperature (15-30°C) for 12 months.

Other Related Reagents
β-mercaptoethanol

Product Description:
TGuide Total RNA Whole Blood Kit is specially designed to extract high
purity RNA from different whole blood samples using TGuide M16
Automated Nucleic Acid Extractor, with no protein and other impurities
pollution. The kit contains reagents and consumables required for
automatic RNA extraction by magnetic bead method, and the reagents are
prepacked in sealed reagent cartridges. Unique embedded magnetic beads
and fully automatic extraction process ensure RNA separation quickly and
conveniently.
RNA isolated by this kit can be used in various downstream experiments
such as RT-PCR, Real Time PCR, chip analysis, Northern blot, Dot blot, polyA
screening, in vitro translation, RNase protection analysis and molecular
cloning without purification.

Product Features
Simple and fast: Ultrapure nucleic acid can be obtained in 73 min.
No contamination:The independent sealed prepacked reagent cartridge
can avoid cross-contamination.
Safe and harmless: The kit and the operation process do not need to use
organic solvents harmful to human body such as phenol and chloroform.

Note: Read this note before using this kit.

1. This kit must be combined with TGuide M16 Automatic Nucleic Acid
   Extractor.
2. This kit is suitable for fresh whole blood samples and blood samples stored
   with RNALock blood RNA stabilizer, but not frozen whole blood samples.
3. Before operation, β-mercaptoethanol is added to Buffer RBB to a final
   concentration of 1%, for example, 10 μl β- mercaptoethanol is added
   to 1 ml Buffer RBB. It is recommended to prepare the lysis buffer right
   before use. The prepared Buffer RBB can be stored at 2-8°C for one
   month. Buffer RBB may form precipitates during storage. If there is any
   precipitate, please heat the buffer to dissolve the precipitate before use.

Preparation of DNase I stock solution:
Dissolve DNase I dry powder (1,500 U) in 550 μl RNase-free ddH2O, mix
gently, and store at -30~-15°C after aliquoting (the stock solution can be
stored for 9 months).
Note: DNase I stock solution thawed from -30~-15°C shall be stored at
2-8°C (can be stored for 6 weeks), and shall not be frozen again.

Preparation of DNase I working solution:
Add 7 volume of Buffer RDD to 1 volume of DNase I stock solution in a new
RNase-free centrifuge tube, and mix gently.

OSR-M610-B-TGuide Total RNA Whole Blood Kit

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