OSR-M301-TGuide Plant Genomic DNA Kit

TGuide Plant Genomic DNA Kit

TGuide Blood Genomic DNA Kit
Cat. No. OSR-M301

Storage Conditions:
It can be stored dry at room temperature (15-30°C) for 12 months.

Product Description:
TGuide Plant Genome DNA Kit is specially designed to extract high purity
DNA from plants using TGuide M16 Automated Nucleic Acid Extractor.
The kit contains reagents and consumables required for automatic DNA
extraction by magnetic bead method, and the reagents are prepacked in
sealed reagent cartridges. Unique embedded magnetic beads, and fully
automatic extraction process are convenient to separate DNA quickly and
conveniently.
The purified genomic DNA can be directly used in PCR, quantitative PCR,
Southern hybridization, RADP/AFLP and other molecular experiments.

Product Features:
Simple and fast: Ultrapure nucleic acids can be obtained in 33 min.
Wide application:Genomic DNA can be extracted from fresh or dried
leaves, seeds and other different plant tissues.
Reliable results: The obtained genomic DNA is free from RNA and protein
contamination and able to be used for PCR or fluorescence quantitative
PCR
Safe and harmless:The kit and the operation process do not need to use
organic solvents harmful to human body such as phenol and chloroform.

Note: Read this note before using this kit.

1. This kit must be combined with TGuide M16 Automatic Nucleic Acid
   Extractor.
2. Repeated freezing and thawing of the sample should be avoided,
   otherwise the extraction yield will be decreased.

Operation steps:

1. Sample treatment:
   Take 50-100 mg of fresh plant tissue or 5-20 mg of dry weight tissue,
   and add liquid nitrogen for full grinding.
   Transfer to a centrifuge tube (not supplied), add 400 μl buffer FP1 and 5
   μl RNase A (10 mg/ml) and mix them by vortex oscillation, and incubate
   at 65°C for 10 min.
2. Add 100 μl buffer FP2, shake well, incubate on ice for 3 min.
3. Add the solution and flocculent precipitate obtained in the previous step
   to a filtration column CS (Place the filtration column in a 2 ml collection
   tube) and centrifuge at 12,000 rpm (~ 13,400× g) for 3 min.
4. Discard the filtration column and retain the filtrate in the collecting
   tube.
5. Transfer about 400 µl of filtrate to a 1.5 ml sample tube.
6. Place the sample tube in the well 4 of the T-rack. Run No.301 program
   (plant genomic DNA extraction program) and only select the final
   elution volume.
   Note: When operating according to the above steps, it is recommended
   to select an elution volume of 100 μl or 150 μl to obtain a higher elution
   concentration.

OSR-M301-TGuide Plant Genomic DNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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