morgan@go2biotech.comNG308-TIANSeq RNA FragcDNA Synthesis Module
TIANSeq RNA Frag/cDNA Synthesis Module
TIANSeq RNA Frag/cDNA Synthesis
Module
Cat. No. 4993005/4993006
Storage Conditions
Please store the kit at -30~-15°C and avoid repeated freezing and thawing.
The shelf life is one year.
Introduction
The TIANSeq RNA Frag/cDNA Synthesis Module is a module of RNA
fragmentation and cDNA synthesis in the non-directional RNA library
construction under the Illumina high-throughput sequencing platform,
which can conduct synthetic reaction of double-strand cDNA. The input
sample suitable for this kit can be both the rRNA-removed total RNA, and
the mRNA directly isolated from total RNAs. This kit includes a complete
set of enzymes and reaction buffers for RNA fragmentation and first- and
second-strand cDNA synthesis. The carefully optimized reaction system has
efficient reverse transcription activity and cDNA synthesis efficiency, and
has good experimental compatibility.
Scope of application: This kit can be applied to the RNA fragmentation and
double-stranded cDNA synthesis reaction of RNA library construction for
the Illumina high-throughput sequencing platform.
Applicable sample input: 10 ng-1 µg total RNAs. As low as 500 pg mRNA of
animals, plants and fungus.
Other recommended reagents
- TIANSeq rRNA Depletion Kit (H/M/R) (4992363/4992364/4992391)
- TIANSeq RNA Clean Beads (4992360/4992362/4992867)
- TIANSeq Size Selection DNA Beads (4992358/4992359/4992979)
Features
Wide range of samples: This kit can be used to conduct fragmentation and
double-strand cDNA synthesis for both mRNA and rRNA-removed RNA.
Efficient conversion:Optimized reaction system to ensure product stability
and efficient double-strand cDNA synthetic efficiency.
Easy and simple operation:Integrated reaction process to simplify
operation steps;
Good compatibility: Purified double-strand cDNA of the reaction product
of the kit can be used directly in the construction of RNA-Seq library.
Precautions Please read the precautions before using this kit.
- Please pay attention to avoiding cross-contamination between nucleic
acid samples and products during operation. - Please use RNase-free tips and centrifuge tubes for the experiments.
- Before the experiment, please clean the bench and treat it with RNase
removal reagent, such as RNase Away (Molecular BioProducts, Inc), to
ensure no RNase contamination. - Before the experiment, please read the instructions carefully. The
samples can be stored at certain steps according to the instructions. - It is recommended to use high-quality RNA samples with RIN value ≥7.0
and good integrity to remove rRNA or isolate mRNA, otherwise, it will
affect the RNA fragmentation and cDNA synthesis efficiency.
NG308-TIANSeq RNA FragcDNA Synthesis Module
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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