KG203-TIANcombi DNA Lyse & Det PCR Kit

TIANcombi DNA Lyse & Det PCR Kit

Fastest DNA Extraction from Plant,Bacteria and Tissue Samples Followed by PCR

TIANcombi DNA Lyse & Det PCR Kit
Cat. No. 4992527/4992528

Compatible device
TGrinder (Cat. No. OSE-Y50)
Storage
Buffer B1 and Buffer B2 can be stored at room temperature (15-30°C) for up
to 12 months without showing any reduction in performance and quality. 2×
Det PCR MasterMix could be stored at -30~-15°C for 12 months, and repeated
freezing-thawing will not affect its activity.

Introduction
TIANcombi DNA Lyse & Det PCR Kit uses special buffer system for fast one-step
DNA extraction and PCR from a wide range of starting materials, including plant
tissues, seeds, animal tissues, blood, yeast and bacteria. The kit contains both
the solution for DNA extraction and the PCR amplification reagent. The whole
extraction process will not include protein, RNA and secondary metabolite
removal step, as well as phenol extraction and ethanol precipitation. Grinding in
liquid nitrogen is also not required.
The 2× Det PCR MasterMix could amplify raw samples with high compatibility,
high efficiency and specificity without removing impurities such as proteins.
This reagent contains TaqDNA polymerase, dNTPs, MgCl2
, buffer, PCR enhancers
and stabilizers, which is fast and simple to use, and especially qualified for highthroughput screening.

Features
Simple and fast: DNA from different tissues can be extracted in 5 min without the
need for liquid nitrogen grinding.
Wide applications: Applicable for plant leaves, seeds, animal tissues, blood samples
(e.g. fresh blood, anti-coagulation blood, blood clots, dried blood spots), yeast and
bacteria, etc.
Good compatibility:The PCR reagents in this kit are suitable for the amplification of
DNA extracted from various sample sources.
Gene detection: Especially suitable for large-scale gene detection.

Important Notes Before Starting

1. Avoid repeated freezing and thawing of the sample, otherwise the extracted
   DNA fragments will be smaller and the extraction yield will be decreased.
2. For phenols-rich samples like cotton leaves, the samples amount should not be
   over 0.4 mg, or it will reduce PCR reaction efficiency.
3. Buffer B1 and Buffer B2 should be stored at room temperature (15-30°C). If a
   precipitate has formed in Buffer, please place the buffer at room temperature
   or warm at 37°C for 10 min to dissolve the precipitate.
4. 2× Det PCR MasterMix provided in this kit is a 2× stock solution. Template,
   primers and sterilized water should be added to make up to a 1× solution
   before use.

Protocol

1. For first use, please check if there is precipitate formed in the two bottles of
   buffer. If there is, please put the buffer at room temperature or 37°C water
   bath until the precipitation is dissolved. The dissolved buffer should be stored
   at room temperature.
2. Put small amount (take table 1 for reference) of samples to 1.5 ml centrifuge
   tube, add 100 µl Buffer B1, and make sure that Buffer B1 could completely
   cover the sample.
3. Grind samples with a grinding pestle.
   Note: For blood or bacteria samples which are difficult to discriminate
   whether have been fully grinded, please grind for 30 sec with grinding pestle.
   And bacteria samples should be centrifuged to collect the cell pellet. For
   plant seeds, skin, connective tissue and etc., please grind it to be muddy with
   grinding pestle (TGrinder (Cat. no. OSE-Y50) will be more convenient to use).
4. Add 100 µl Buffer B2, vortex to mix, then centrifuge at 12,000 rpm (~13,400 x g)
   for 2 min.
5. Pipet 100 µl supernatant to a clean 1.5 ml centrifuge tube to be used as
   template.
6. Set up the PCR amplification reaction.

Result Detection
Take 5-10 µl reaction products for agarose electrophoresis detection.
Note: the example provided is for your reference. As the template
and primers differs, the real reaction condition should be modified
accordingly.

KG203-TIANcombi DNA Lyse & Det PCR Kit

All trademarks or registered trademarks appearing on this website are the property of their respective owners.

This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

Need more info ? Contact us anytime. We’re here: Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017