KT109-Multi PCR Kit

Multi PCR Kit
Cat. No. 4992787/4992788
Storage: -30~-15°C for one year.

Introduction
This kit is specially developed for fast and efficient multiplex PCR. Among all of the HotStart DNA
polymerases found till now, Multi HotStart DNA Polymerase shows the best performance. The chemically
modified enzyme should be heated at 95°C for 15 min to fully release the activity, so as to ensure high specificity.
The hot-start feature enables reactions to be set up at room temperature. The PCR buffer contains a balanced combination of salts and additives which enable comparable efficiencies for annealing and extension of all primers in the reaction which is rapid and convenient.
For complex templates, Mg2+ concentration could be adjusted to get optimized result.

Unit Definition
One unit of Multi HotStart DNA Polymerase is defined as the amount that incorporates 10 nmol of dNTPs into acid-insoluble substrates within 30 min at 74°C with activated salmon sperm DNA as the template-primer.

Storage Buffer
20 mM Tris-HCl (pH9.0), 0.1 mM EDTA, 1 mM DTT, 100mM KCl, Stabilizers, 50% Glycerol (v/v)

Applications
1.Multiplex PCR
2.Amplification of highly specific DNA
3.Amplification of low copy template
4.Amplification of complex template (e.g.complex
genomic DNA, cDNA, etc.)

Product Highlights
1.High specificity:Chemically modified HotStart polymerase ensures highly specific amplification.
2.High sensitivity:Low copy amplification and high efficiency of multiplex PCR can be realized.
3.Simple operation: The enzyme has no activity at low temperature and room temperature, and the reaction can be prepared at room temperature.

Example
Note: The following example provided is only for reference, user must set up optimal reaction system
according to different reaction conditions such as different templates or primers, etc.

KT109-Multi PCR Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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