MD102-DNA Marker II

DNA Marker II
Cat. no. 4992937/4992938
Storage: -30~-15°C for 2 years. For frequently use, it
can be stored at 2-8°C for 3 months.
Concentration: About 0.05 mg DNA/ml

Description
DNA Marker II consists of six double-stranded DNA bands
that are suitable as molecular weight standards for agarose gel electrophoresis.
The product is ready-to-use; it is premixed with 1× Loading Buffer for direct loading 3~6 μl on agarose gel electrophoresis.
The ladder is composed of six individual DNA fragments (in base pairs): 1200, 900, 700, 500, 300, and 100. It contains one intense band (700 bp) for easy orientation.
To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.

Buffer Contents
10 mM Tris-HCl (pH 8.4)
10 mM EDTA
0.02% Bromophenol Blue
5% Glycerol

Protocol

1. Directly load 3~6 μl of the product to the agarose gel(load 1 μl product per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).
2. Electrophoresis condition: 1.0~2.5% agarose gel, 4~10 v/cm voltage.
3. DNA marker can be visualized using ethidium bromide under UV light.

Recommendations

1. Directly use, do not heat before loading.
2. Please use new agarose gel and change electrophoresis
   buffer in time, so as not to affect the electrophoresis results.