FastKing One Step RT-PCR Kit-GKR123

.. 104 / Publication

More efficient and sensitive one-step RT-PCR reagent

Catalog number / packaging

 Manual Inquire

• StorageStore at -20℃.
• DescriptionThe FastKing One Step RT-PCR Kit adopts the one-step method to enable RT and PCR to be carried out in the same reaction system. No reagent needs to be added and the tube cover does not need to be opened in the reaction process, thus avoiding cross contamination between samples and improving the detection sensitivity. The 25×RT-PCR Enzyme Mix in the kit is a premixed mix form of TIANGEN novel reverse transcriptase (King RTase), antibody modified hot-start Taq DNA polymerase and RNase Inhibitor. It has stronger RNA affinity and thermal stability, extension capability to complex secondary structure RNA templates, and higher amplification efficiency and specificity to cDNA after reverse transcription. In addition, the 2× FastKing One Step RT-PCR MasterMix in this product is a new type of reaction system specially optimized for the above two key enzymes, which contains necessary ionic components, dNTPs, PCR stabilizers and enhancers. It ensures that King RTase and Taq polymerases have the best performance in the whole one-step reaction process.
• Features■ Purity: Reverse transcription and PCR reactions are completed in one step to avoid cross contamination. ■ High Efficiency: Unique King reverse transcriptase with RT efficiency over 95%. ■ Sensitive: As low as 1 ng templates can be accurately identified, especially for templates with low abundance. ■ Specificity: The antibody-modified Taq polymerase further improves the amplification efficiency and specificity.
• ApplicationsIt is suitable for detecting gene expression level in cells and tissues, cloning cDNA of specific genes and detecting RNA virus. It is especially suitable for qualitative detection of low abundance templates.

Publication

.. 104 / Publication

Sort by

Impact Factor

Journals

–

• Dimethylglycine Alleviates Metabolic Dysfunction-Associated Fatty Liver Disease by Improving the Circulating Estrogen Level via Gut Staphylococcus .Journal of agricultural and food chemistry2024-02-08 Read ArticleAbstract: Our previous study screened out dietary 0.1% dimethylglycine (DMG); which had beneficial effects on egg production and fat deposition in laying hens during the late laying period.. In this paper; it was further found that dietary DMG alleviated fatty liver disease and enhanced lipid deposited into the yolk while promoting hepatic lipid transport.. There are intestinal estrogen-metabolizing bacteria (EBM) having ??-glucuronase (GUS) activity that regulates the content of circulating estrogen (E2) in mammals.
• Metformin enhances METTL14-Mediated m6A methylation to alleviate NIT-1 cells apoptosis induced by hydrogen peroxideHeliyon2024-01-17 Read Article.. cDNA according to the instructions of the kit (KR123; TIANGEN; Beijing; China). qRT-PCR testing was conducted based on
• A variant of the venom allergen-like protein, Dd VAP2, is required for the migratory endoparasitic plant nematode Ditylenchus destructor parasitism of plantsFrontiers in Plant Science2023-12-13 Read Article.. USA); and then reverse transcribed into cDNA using the FastKing One Step RT-PCR kit (Tiangen; Beijing; China).
• Targeting the E2F1/Rb/HDAC1 axis with the small molecule HR488B effectively inhibits colorectal cancer growthCell Death & Disease2023-12-07 Read ArticleSYBR?? Green Real-time PCR Master Mix (KR123; TIANGEN; China).
• Self-assembled miR-134-5p inhibitor nanoparticles ameliorate experimental bronchopulmonary dysplasia (BPD) via suppressing ferroptosisMikrochimica Acta2023-11-30 Read Article.. carried out using Tiangen FastKing OneStep qRT-PCR Kit (KR123; Tiangen Biotech). qRT-PCR was conducted on

FastKing One Step RT-PCR Kit

Need more info ? Contact us anytime. We’re here:
Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
Address: Rm 4L, Golden Century Building, Shennan Av. 6033#, Shenzhen, China