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Sensitive and stress-resistant special fluorescent quantitative reagent for lncRNA

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• StorageStore at -20°C
• DescriptionCompared with mRNA, lncRNA has the characteristics of low abundance, large difference in GC content and more complex secondary structure, and traditional quantitative reagents are difficult to achieve ideal effects on lncRNA. The 2×lnR lncRNA PreMix in this kit is a new generation of pre-mixed real time PCR detection reagent specially developed for quantitative detection of lncRNA. The antibody modified hot-start DNA Polymerase ensures that the product has higher detection sensitivity while ensuring higher reaction specificity. In addition, the addition of H-competitor factor and EP component in Buffer makes the product have a wide range of sample universality. It has very good amplification applicability for templates with different GC contents, complex advanced structures, more PCR inhibitor residues and long fragments, etc., which is especially suitable for quantitative detection of lncRNA with relatively complex advanced structures and relatively low overall abundance.
• Features■ Highly sensitive antibody modified polymerase, suitable for fast quantitative workflow and can efficiently complete lncRNA detection.■ The H-Competitor factors compete for hydrogen bonds, perfectly amplifying high GC and complex lncRNA templates.■ The EP component stabilizes PCR system, effectively protects enzyme activity and resists interference of various inhibitors.
• ApplicationsIt is especially suitable for experiments of lncRNA expression analysis by SYBR Green method on various real time PCR instruments, and is also suitable for quantification of templates with high GC, complex secondary structure, high impurity residue and long fragment cDNA.

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• The proposed role of MSL-lncRNAs in causing sex lability of female poplars.Horticulture research2023-05-01 Read Article.. April 2024 by using an lnRcute lncRNA qPCR Kit (Tiangen Co. Ltd; Nanjing; China); with the Actin2
• Pan-cancer analysis of PCAT6 and its effect on oesophageal squamous cell carcinoma cell proliferation and migration.Medical oncology (Northwood, London, England)2023-03-24 Read ArticleAbstract: Bioinformatics methods were used to analyze the role of PCAT6 in a variety of tumors and verify its role in oesophageal squamous cell carcinoma (ESCC) EC109 cells.. The pan-cancer dataset was downloaded from the University of California Santa Cruz (UCSC) database to analyze the expression of PCAT6 in pan-cancer and its relationship with prognosis; clinical features; and immune infiltration.. The expression and prognosis of PCAT6 in ESCC were verified by Gene Expression Omnibus (GEO) and Kaplan??Meier database.
• Identification of a cellular senescence-related-lncRNA (SRlncRNA) signature to predict the overall survival of glioma patients and the tumor immune microenvironmentFrontiers in Genetics2023-02-24 Read ArticleThe lnRcute lncRNA qPCR Kit (FP402; Tiangen Biotech Co.; Ltd.) was used to perform RT-qPCRs according to the manufacturer??s instructions.
• The Association between the Differential Expression of lncRNA and Type 2 Diabetes Mellitus in People with HypertriglyceridemiaInternational Journal of Molecular Sciences2023-02-21 Read Article.. cDNA was then analyzed by RT-qPCR using lnRcute lncRNA qPCR Kit (FP402; TIANGEN) or SuperReal PreMix Plus (SYBR Green) (FP205;
• Exosomes from high glucose-treated macrophages promote epithelial??mesenchymal transition of renal tubular epithelial cells via long non-coding RNAsBMC Nephrology2023-01-30 Read Article.. carried out using a lncRNA qPCR Detection Kit (Tiangen; China) and Fast SYBR

lnRcute lncRNA qPCR Kit (SYBR Green)

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