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High-efficiency miRNA extraction kit specially targeting plant samples

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• StorageStore at room temperature (15-25°C).
• DescriptionThis kit can rapidly extract total RNA containing miRNA from plant tissues, especially plant tissues rich in polysaccharide, polyphenol or starch (such as cotton leaves, potato tubers, apples, peach tree leaves, etc.), and can simultaneously process a large number of different samples. The extracted total RNA has high purity and is free of protein and other impurities.
• Features■ The lysis buffer is specially optimized and equipped with sedimentation aid solution PLA, which is specially designed for the lysis of polysaccharide polyphenol plant samples with higher yield.■ Column purification, free from impurity pollution, higher purity, suitable for downstream quantitative detection.■ Comprehensive functions to extract total RNA in addition to small fragments.■ The extraction can be completed within 1 h, improving the extraction efficiency.
• ApplicationsIt can be used in RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.

Publication

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• Protective effect of low??intensity pulsed ultrasound on immune checkpoint inhibitor-related myocarditis via fine-tuning CD4 + T-cell differentiationCancer Immunology, Immunotherapy2024-01-18 Read Article.. CD4 + T-cells was isolated using a RNeasy Isolation Kit (Tiangen Biotech; Beijing; China); and then the miRcute Plus
• MicroRNA-encoded regulatory peptides modulate cadmium tolerance and accumulation in rice.Plant, cell & environment2024-01-17 Read ArticleAbstract: Key Laboratory of Ministry of Education for Genetics; Breeding and Multiple Utilization of Crops; College of Agriculture; Fujian Agriculture and Forestry University; Fuzhou; China Key Laboratory of Biological Breeding for Fujian and Taiwan Crops; Ministry of Agriculture and Rural Affairs; Key Laboratory of Crop Biotechnology of Fujian Higher Education Institutes; Fujian Agriculture and Forestry University; Fuzhou; China. Genetics; Breeding and Multiple Utilization of Crops; College of Agriculture; Fujian Agriculture and Forestry University; Fuzhou; China 2Key Laboratory of Biological Breeding for Fujian and Taiwan Crops; Ministry of Agriculture and Rural Affairs; Key Laboratory of Crop Biotechnology of Fujian Higher Education Institutes; Fujian Agriculture and Forestry University; Fuzhou; China Correspondence Yuanyuan Song and Rensen Zeng; Key Laboratory of Ministry of Education for Genetics; Breeding and Multiple Utilization of Crops; College of Agriculture; Fujian Agriculture and Forestry University; Fuzhou 350002; China.. Email: yyuansong@fafu.edu.cn and rszeng@fafu.edu.cn Funding information National Key R&D Program; Grant/Award Number: 2023YFC2812300; National Natural Science Foundation of China; Grant/Award Numbers: 32101676; U2005208; 31971833; 32371588; Natural Science Foundation of Fujian Province; Grant/Award Numbers: 2021J05019; 2021J01075; 2021J02024; Fujian Agriculture and Forestry University Natural Science Funds for Distinguished Young Scholar; Grant/Award Number: xjq21001 MicroRNAs (miRNAs) are small noncoding RNAs that play a vital role in plant responses to abiotic and biotic stresses.
• Mulberry-derived miR168a downregulates BmMthl1 to promote physical development and fecundity in silkworms.International journal of biological macromolecules2024-01-08 Read ArticleAbstract: Plant-derived miRNAs and their interactions with host organisms are considered important factors in regulating host physiological processes.. In this study; we investigated the interaction between the silkworm; an oligophagous insect; and its primary food source; mulberry; to determine whether mulberry-derived miRNAs can penetrate silkworm cells and regulate their functions.. Our results demonstrated that miR168a from mulberry leaves enters the silkworm hemolymph and binds to the silkworm Argonaute1 BmAGO1; which is transported via vesicles secreted by silkworm cells to exert its regulatory functions.
• Multi-omics analysis of small RNA, transcriptome, and degradome to identify putative miRNAs linked to MeJA regulated and oridonin biosynthesis in Isodon rubescens.International journal of biological macromolecules2023-12-30 Read ArticleAbstract: Isodon rubescens has garnered much attention due to its anti-tumor or anti-cancer properties.. However; little is known about the molecular mechanism of oridonin biosynthesis leveraging the regulatory network between small RNAs and mRNAs.. In this study; the regulatory networks of miRNAs and targets were examined by combining mRNA; miRNA; and degradome.
• Endophytic fungus Penicillium steckii DF33 promoted tanshinones biosynthesis in Salvia miltiorrhiza by regulating the expression of CYP450 genes.Gene2023-12-22 Read ArticleAbstract: Salvia miltiorrhiza; a prominent traditional Chinese medicinal resource; has been extensively employed in the management of cardiovascular and cerebrovascular ?? These authors contributed equally to this work.. ??Correspoding authors at: Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province; College of Life Sciences and Medicine; Zhejiang Sci-Tech University; Hangzhou 310018; Zhejiang; China (H. Chen and Z. Liang).. F-mail addresses: chenhm@zstu.edu.cn(H. Chen); liangzs@zstu.edu.cn (Z. Liang)

miRcute Plant miRNA Isolation Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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