go2biotech – Reagents and instruments for immunology, cell biology and molecular biology.

Highly efficient extraction of RNA > 18 nt from paraffin sections

Catalog number / packaging

 Manual Inquire

• StorageDNase I, buffer RDD and RNase-Free ddH₂O (tube) should be stored at 2-8°C. Other reagents should be stored at room temperature (15-25°C).
• DescriptionFormalin fixation can lead to cross-linking of RNA-RNA and RNA- protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the crosslinking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.
• Features■ High specificity: Specially designed for extracting miRNA from FFPE with optimized components.■ Simple and fast: High-quality miRNA can be obtained within 2 hours.■ Ultra-pure: Column purification to avoid gDNA and salt contamination.
• Required ReagentsXylene, anhydrous ethanol
• Applications■ RT-PCR■ RT-qPCR■ Northern Blot analysis■ Microarray analysis■ Sequencing of miRNA library

Publication

.. 123 / Publication

Sort by

Impact Factor

Journals

–

• Expression of miRNA-29c in the carotid plaque and its association with diabetic mellitusFrontiers in Cardiovascular Medicine2024-02-05 Read Article.. specimens was isolated by a DP502 reagent kit following manufacturer’s instruction (TIANGEN Inc.; Beijing; P.R.
• Unveiling 2,000??years of differentiation among Tungusic-speaking populations: a revised phylogeny of the paternal founder lineage C2a-M48-SK1061Frontiers in Genetics2023-07-24 Read Articlersity and Inner Mongolian Normal University; and complied with the ethical principles of the 2013 Helsinki Declaration of the World Medical Association. Genomic DNA was extracted using the DP-318 Kit (Tiangen Biotechnology; Beijing) according to the manufacturer??s protocol. Firstly; Routine Y-SNP tests were done to determine the general haplogroup of all male samples. And then DNA specimens extract
• Genetic Analysis of Orf Virus (ORFV) Strains Isolated from Goats in China: Insights into Epidemiological Characteristics and Evolutionary PatternsVirus Research2023-07-05 Read ArticleViruses were isolated as described previously ( Wang??et??al.; 2019 ); and isolates were named FX and LX; respectively. The genomic DNA of ORFV was obtained using the Tianamp Genomic DNA Kit (DP-304??02; TIANGEN; China) and used as templates for gene amplification. Specific primers for B2L; F1L; VIR; ORF109; GIF; VIL-10 and ORF125 were designed by SnapeGene software (v4.2.4) according to the ORFV refe
• Integrated Transcriptomics and Metabolomics Analysis of the Fructan Metabolism Response to Low-Temperature Stress in GarlicGenes2023-06-19 Read Articlesamples were stored at ???80 ??C immediately after being frozen in liquid nitrogen. The total RNA of garlic samples was extracted according to the instructions of the TRNZOL Extraction Reagent (DP 424; Tiangen Biochemical Technology Ltd.; Beijing; China). The integrity of the RNA was detected using 1% agarose gel electrophoresis. The quality of the total RNA was detected using a TGem microspectropho
• NFAT5 Restricts Bovine Herpesvirus 1 Productive Infection in MDBK Cell Cultures.Microbiology spectrum2023-05-25 Read Article(2-DDCT) by comparison to the control. DNA purification and quantification of viral DNA by qPCR. Total genomic DNA was extracted from infected cells via using a commercial viral DNA purification kit (Tiangen; cat# DP-348) following the manufacturer’s instructions. Freshly prepared DNA was used as a template for real-time qPCR to measure viral DNA levels with gC-specific primers; as described above
• miRNAprep Pure FFPE Kit

All trademarks or registered trademarks appearing on this website are the property of their respective owners

This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

Need more info ? Contact us anytime. We’re here:
Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017
Address: Rm 4L, Golden Century Building, Shennan Av. 6033#, Shenzhen, China