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Professional reagent for high resolution melting curve analysis

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• StorageStore in dark at -20℃.
• DescriptionThis product combines the advantages of saturated dye EvaGreen and antibody modified polymerase. It is a professional kit suitable for High Resolution Melting (HRM) analysis. This product is 2×PreMix, and the preparation of PCR reaction solution is very simple and convenient. It has the characteristics of high resolution and high adaptability of templates.Different from SYBR Green, EvaGreen does not inhibit PCR reaction at high concentration. The binding of double-stranded PCR products with EvaGreen dye is saturated, so it is called Saturated Dye. The Dye Rearrangement phenomenon of SYBR Green will not occur and the difference of single base between amplification products can be well distinguished. The product can be used for analysis of known SNPs, screening of unknown SNPs, scanning of unknown mutant genes, methylation PCR analysis, etc.
• Features■ High resolution: Adopt EvaGreen saturated dyes, which has high resolution of melting curve in saturated state and can distinguish the variation of single base.■ High specificity: Use antibody modified hotstart DNA polymerase to reduce non-specific amplification and improve specificity.■ High stability: The carefully optimize Buffer system increase the stability of melting curve and improve the reliability of results.■ ROX correction: ROX dye is packaged separately, which is more flexible to use and have more accurate results.
• Applications■ Known SNP typing.■ Unknown SNP screening.■ Unknown mutant genes scanning.■ Methylation PCR analysis.

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• Meta-data analysis of kidney stone disease highlights ATP1A1 involvement in renal crystal formationRedox Biology2023-02-27 Read Article.. plate by using the HRM analysis kit (Tiangen; China) according to the
• CRISPR/Cas9-mediated F1534S substitution in the voltage-gated sodium channel reveals its necessity and sufficiency for deltamethrin resistance in Aedes albopictusJournal of Pest Science2022-08-22 Read Article.. assay (HRMA) was conducted with an HRM Analysis Kit (EvaGreen; Tiangen; Beijing; China) on a LightCycler??96 System
• Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed Sheep TBXT HeterozygotesAnimals : an Open Access Journal from MDPI2022-03-21 Read ArticleUsing an HRM analysis kit (Tiangen; Beijing; China); the total reaction volume was 10 ??L; including
• Downregulation of the circadian rhythm regulator HLF promotes multiple-organ distant metastases in non-small cell lung cancer through PPAR/NF-??b signaling.Cancer letters2021-01-05 Read ArticleAbstract: Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death due to its early recurrence and widespread metastatic potential.. Accumulating studies have reported that dysregulation of circadian rhythms-associated regulators is implicated in the recurrence and metastasis of NSCLC.. Therefore; identification of metastasis-associated circadian rhythm genes is clinically necessary.
• Construction of an efficient genomic editing system with CRISPR/Cas9 in the vector mosquito Aedes albopictus.Insect science2019-08-05 Read ArticleHRMA was conducted with an HRM Analysis Kit (EvaGreen; TIANGEN) on a This article is protected by copyright.

HRM Analysis Kit (EvaGreen）

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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