miRcute Plant miRNA Isolation Kit-Tiangen OSE- ( Go2biotech.com – Distribution for Reagents and instruments )

Introduction
This kit can rapidly extract total RNA containing miRNA from plant tissues,
especially plant tissues rich in polysaccharide and polyphenol or starch
(such as cotton leaves, potato tubers, apples, peach tree leaves, etc.),
and can simultaneously process a large number of different samples.
The extracted total RNA has high purity and is free from protein and
other impurities contamination. It can be used in various downstream
experiments such as RT-PCR, Real Time RT-PCR, chip analysis, Northern
Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection
analysis, molecular cloning etc.
To avoid RNase contamination, please note that:

1. Change new gloves frequently, because the skin contains bacteria and
   may cause RNase contamination.
2. Use RNase-free plastic products and pipette tips to avoid cross
   contamination.
3. RNA will not be degraded by RNase in Buffer SLM. However, plastic and
   glassware without RNase should be used in the subsequent processing.
4. RNase-Free ddH2O should be used for the preparation of solutions (add
   water into a clean glass bottle, add DEPC to a final concentration of
   0.1%(v/v), mix well, place overnight, and autoclave).
   Precautions Please read these precautions before use
5. Before operation, add β-mercaptoethanol to Buffer SLM to a final
   concentration of 5%. For example, add 25 μl of β-mercaptoethanol
   to 475 μl of Buffer. It’s better to prepare the reagent when using. The
   prepared reagent can be stored for one month at 2-8°C.
6. Add 96-100% ethanol to Buffer MRD and Buffer RWA before the first
   use. Please refer to the label on the bottle for the added volume.
7. Carry out all extraction operations at room temperature.

miRcute Plant miRNA Isolation Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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