TGuide Smart Envir-DNA Kit-Tiangen OSE- ( Go2biotech.com – Vertrieb für Reagenzien und Instrumente)
Introduction
This kit uses a unique lysis solution combined with a specially optimized and highly
efficient HA removal buffer to extract genomic DNA of Gram-negative bacteria, Grampositive bacteria, fungi and other microorganisms from a variety of environmental
samples, such as soil, feces, fermentation products, water, pond sludge, marine
sediments, and intestinal contents. The grinding beads and homogenizer can
effectively crush the microorganisms in the environmental samples to ensure the
high efficiency and integrity of the extracted genomic DNA.
Genomic DNA purified by this kit has few impurities and good integrity, and can be
directly used in downstream experiments of molecular biology such as PCR, library
prep and NGS.
Features
Quick and easy: Quickly remove humic acid in 1 min.
High purity: Combined with magnetic bead purification, the extracted DNA has high
purity and can be directly used in downstream experiments.
Wide application: Can be applied to microbial genomic DNA extraction for many
types of samples.
Good compatibility: It can be applied to a wide range of TGuide S16/S32/S32 Pro
automated nucleic acid extractor and can be extended for use with the TGuide S96
Dex Automated Nucleic Acid Extractor.
Notes Please be sure to read this precaution before using the kit.
- The newly collected samples will get higher yield, and different samples should
consult the corresponding optimal preservation conditions before sampling. - Excess DNA may inhibit downstream PCR reactions, and it is recommended to
dilute the DNA template for use if inhibition appeared. - HA Removal Buffer CN needs to be pre-cooled at 2-8°C before use in order to
achieve its optimal humic acid removal effect. - Buffer TB does not contain EDTA. In order to minimize the degradation of the
extracted nucleic acids for long time storage, customer can replace the Buffer
TB with the EDTA-containing Buffer TE if necessary, but pay attention to the
fact that the EDTA will affect the downstream experiments, such as PCR, library
construction, and so on. - Due to the complexity of environmental samples, which usually have more
impurities, the concentration of extracted nucleic acids is low, and degradation of
nucleic acids may occur in long time storage, etc. It is recommended to carry out
downstream experiments as soon as possible after extraction.
TGuide Smart Envir-DNA Kit
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Dieses Produkt ist ausschlie?lich für wissenschaftliche Forschungszwecke bestimmt. Nicht in der Medizin, bei klinischen Behandlungen, Lebensmitteln oder Kosmetika verwenden.
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