morgan@go2biotech.comRNAprep Pure Micro Kits – ( Tiangen GDP420 ) ( Go2biotech.com – Distribution for Reagents and instruments )
For purification of high-quality total RNA from micro amount of tissues or cells
RNAprep Pure Micro Kits – ( Tiangen GDP420 ) Catalog number / packaging
| Mat. No | Ref. No | No. of preps |
| 4992859 | Gdp420 | 50 |
| RNAprep Pure Micro Kit |
| Cat. no. GDP420 |
| (Spin Column) |
| DP240514 |
| Kit Contents |
| 1 |
| Compatible Reagents |
| Proteinase K (Cat. no. GRT403-01/GRT403-02), Grinding Pestles, RNasefree Columns CS set |
| Storage |
| RNAprep Pure Micro Kit can be delivered at room temperature (15-30°C). |
| When the kit has arrived, store RNase-free DNase I, Buffer RDD, and |
| RNase-free ddH2O at 2-8°C for up to 15 months. Other reagents can be |
| stored at room temperature (15-30°C) for 15 months. |
| Contents GDP420 |
| (50 preps) |
| Buffer RL 30 ml |
| Buffer RW1 40 ml |
| Buffer RW 12 ml |
| GDP420H |
| Carrier RNA 310 µg |
| RNase-Free ddH2O 1 ml |
| RNase-Free ddH2O 2×15 ml |
| (RNase-Free Columns CR1 set) 50 |
| RNase-Free Centrifuge Tubes (1.5 ml) 50 |
| Handbook 1 |
| RNase-Free DNase I (1500 U) 1 |
| GRT411 Buffer RDD (DNA Digest Buffer) 4 ml |
| RNase-Free ddH2O 1 ml |
| Note: GDP420H, GRT411 are shipped and packaged separatly. |
Storage
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃
Other reagents could be store at room temperature (15-25℃)
The function of carrier RNA: A special carrier RNA is provided in the kit. When RNA is extracted from a very small amount of sample, it is recommended to add Carrier RNA in the system, which helps to improve the binding of RNA to adsorption column.
Description
The RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is 200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.
The RNAprep Pure Micro Kit uses a highly efficient, nucleic acid-specific centrifugal adsorption column and a unique buffer system to rapidly extract total RNA from a wide range of different types of microsamples. This kit includes Carrier RNA, which can easily capture trace nucleic acids from the system. The extraction of RNA by this kit is convenient, rapid and reproducible with high yield. The reaction can be completed within 30-40 minutes. The kit can selectively remove all RNA that is 200 nt. The extracted total RNA is extremely pure and free of DNA and protein contamination.
Required Reagents
β-mercaptoethanol, ethanol
Features
■ Capable of purifying high-quality RNA from trace amount samples, such as micro-dissected tissue, fibrous tissue and cells.
■ Unique DNase I minimizes genomic DNA contamination.
■ The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.
■ No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.
Applications
■ RT-PCR.
■ Northern Blot, Dot Blot.
■ Real-Time PCR.
■ Chip analysis.
■ PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.
RNAprep Pure Micro Kits – ( Tiangen GDP420 ) Manual
RNAprep Pure Micro Kits – ( Tiangen GDP420 ) Publication
A Bursaphelenchus xylophilus effector BxICD1 inducing plant cell death, concurrently contributes to nematode virulence and migration
Frontiers in Plant Science2024-02-28 Read Article
.. Total RNA was extracted using the RNAprep Pure Micro Kit (DP420; TianGen Biotech); and first-strand cDNA was synthesized from
Glucocorticoids increase adiposity by stimulating Kr??ppel-like factor 9 expression in macrophages
Nature Communications2024-02-08 Read Article
ATMs RNA was collected from the sorted cells via RNAprep pure Micro Kit (TIANGEN).
Human Fallopian Tube-Derived Organoids with TP53 and RAD51D Mutations Recapitulate an Early Stage High-Grade Serous Ovarian Cancer Phenotype In Vitro.
International journal of molecular sciences2024-01-24 Read Article
RNA extraction was performed by using an RNAprep Pure Micro Kit (DP420; Tiangen; Beijing; China).
KAS-seq profiling captures transcription dynamics during oocyte maturation
Journal of Ovarian Research2024-01-24 Read Article
.. of 50 oocytes from the specified experimental group; employing the RNAprep Pure Micro Kit (TIANGEN DP420).
The Effects of Nine Compounds on Aldehyde-Oxidase-Related Genes in Bactrocera dorsalis (Hendel).
Genes2024-01-24 Read Article
.. trace sample total RNA extraction kit was used (DP420; TIANGEN BIOTECH Co.; Ltd.; Beijing; China); as well as a Quant
RNAprep Pure Micro Kits – ( Tiangen GDP420 ) Manual
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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