EndoFree Mini Plasmid Kit II(Tiangen GDP118) ( Go2biotech.com – Distribution for Reagents and instruments )

For purification of endotoxin-free transfection grade plasmid DNA

EndoFree Mini Plasmid Kit II(Tiangen GDP118) Catalog number / packaging

Description

The EndoFree Mini Plasmid Kit II adopts a unique silica membrane adsorption technology to efficiently and specifically bind plasmid DNA. The special endotoxin-removing system can effectively remove impurities such as endotoxin and proteins. The whole extraction process takes only 1 hour, which is convenient and quick. The plasmid extraction yield is related to the plasmid copy number, host bacteria type and the culture conditions. Up to 70 μg of plasmid DNA can be extracted from 5-15 ml of the overnight cultured bacteria culture medium.

Features

■ Rapid and high yield: 5-70 μg plasmid DNA can be extracted in around 1 hour.

■ High-purity: The special endotoxin removal system combined with Spin Column CP4 can specifically adsorb nucleic acid and remove endotoxin to the minimal level.

■ Wide range of applications: Suitable for routine experiments such as restriction endonuclease digestion, PCR, sequencing, ligation, transformation, as well as the high-precision experiments such as gene therapy, cell microinjection, gene silencing, transcription, etc.

Required Reagents

Ethanol, isopropanol

Storage

Room temperature (15-25℃ )

Plasmid Yield

EndoFree Mini Plasmid Kit II(Tiangen GDP118) Publication

• RGD-p21Ras-scFv expressed prokaryotically on a pilot scale inhibits ras-driven colorectal cancer growth by blocking p21Ras-GTPBMC Cancer2024-01-12 Read Article.. standard experimental step using the EndoFree Mini Plasmid Kit II (Cat. #DP118-02) from TIANGEN BIOTECH (Beijing) Co.; Ltd.; and stored

• Regulation of epithelial cell differentiation by the Ubiquitous expressed transcript isoform 1 in ulcerative colitis.Journal of gastroenterology and hepatology2023-11-27 Read ArticleAll plasmids were isolated using endotoxin-free plasmid extraction kit (Tiangen; DP118-02) and confirmed through DNA sequencing.

• CHD1L knockdown suppresses bladder cancer cell growth in vitroEurope PMC2023-10-28 Read ArticleThe selected high purity plasmids containing interference sequence were extracted by EndoFree midi Plasmid Kit (Cat. #DP118-2; TIANGEN).

• S -acylation of p62 promotes p62 droplet recruitment into autophagosomes in mammalian autophagyMolecular Cell2023-10-05 Read ArticleEndoFree Mini Plasmid kit II ; TIANGEN ; Cat#DP118-02.

EndoFree Mini Plasmid Kit II(Tiangen GDP118) Manual

 Manual

EndoFree Mini Plasmid Kit II(Tiangen GDP118)

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