Mouse Tissue Direct PCR Kit(Tiangen GKG205)( Go2biotech.com – Distribution for Reagents and instruments )

Rapid amplification of the target gene directly from animal tissue samples without extraction

Mouse Tissue Direct PCR Kit(Tiangen GKG205)

Catalog number / packaging

 Manual

Storage
The Tissue Lysis Buffer and Digestive Enzyme should be stored under room temperature (15-25℃ ). The 2×Dir PCR MasterMix should be stored at -20℃ .

Description
The Mouse Tissue Direct PCR Kit adopts a unique packaging design that includes all the reagents for rapid genomic DNA preparation and PCR amplification. It is applicable for the one-step genome DNA purification from mouse tissues (tail, ear and toe) and the subsequent PCR amplification and detection. The whole process does not include homogenization, smashing, overnight digestion, organic solvent extraction, ethanol precipitation or column purification steps. Stable results can be obtained with simple and fast operations.

The 2× Dir PCR MasterMix provided by this kit is a highly compatible PCR reagent that can efficiently and specifically amplify DNA without the need for removing impurities such as proteins. This reagent contains an antibody modified hot-start Taq DNA polymerase, dNTPs, MgCl2, buffers, as well as the enhancer, optimizer and stabilizer for PCR reaction. The PCR reaction can be performed by simply adding in roughly extracted template and specific primers. The whole process is fast, simple, sensitive, specific and stable, which is especially suitable for high-throughput screening. The 2× Dir PCR MasterMix contains premix electrophoresis dye, so that the PCR products can be directly sent for electrophoresis detection after the reaction. The 3’ end of the PCR product has a A-tailing, which can be used for TA cloning.

Features
■ Simple and fast: Genomic DNA can be rapidly extracted from mouse tissues in 60 minutes without liquid nitrogen grinding and organic solvent extraction.
■ Wide application: It is suitable for one-step extraction of genomic DNA from mouse tail, ear, toe and other tissues.
■ High specificity: The Taq polymerase used in this product is an antibody modified hot-start enzyme, with high template and primer affinity and amplification specificity, which is especially suitable for genotyping and transgenic identification.
■ Gene detection: The product is easy to operate with reliable results, and it is especially suitable for high-throughput analysis and detection of mouse tissues.

Workflow

Mouse Tissue Direct PCR Kit(Tiangen GKG205) Publication

• Disruption in CYLC1 leads to acrosome detachment, sperm head deformity, and male in/subfertility in humans and micemedRxiv2024-01-12 Read ArticlePrePrint: .. PCR and/or sequencing of tail genomic DNA using the Mouse Tissue Direct PCR Kit (Tiangen Biotech; Beijing; China).
• CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men.EBioMedicine2023-07-17 Read Article.. with genotyping primers (Table S2) using the Mouse Tissue Direct PCR Kit (Tiangen Biotech; Beijing; China).
• A cervical cancer tissue-derived decellularized extracellular matrix scaffold for cervical cancer tissue reconstruction in vitroJournal of Southern Medical University2023-02-20 Read Article). DNA quantifications We assessed the content of genetic material in fresh tissues and decellularized ECM scaffold. Briefly; 10 mg of each sample ( n =6) was digested with 200 ??L proteinase K (RT403; Tiangen; China) at 55 ??? for 3 h; and the total genomic DNA was isolated using TIANcombi DNA Lyse & Det PCR Kit (KG203; Tiangen; China) following the manufacturer’s instructions. The relative DNA conte
• Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathwayFrontiers in Surgery2023-01-16 Read Article.. Total RNAs were extracted by animal tissue/cell total RNA extraction kit (Tiangen; China); and 500 ng total

Mouse Tissue Direct PCR Kit(Tiangen GKG205)

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