TIANScriptⅡ RT Kit(Tiangen GKR107)( Go2biotech.com – Distribution for Reagents and instruments )

Suitable for templates with complex secondary structures and efficient synthesis of long-chain cDNA

TIANScriptⅡ RT Kit(Tiangen GKR107)

Catalog number / packaging

 Manual

Storage
Store at -20°C.
Description
The kit provides all reagents for synthesizing the first strand of cDNA, wherein TIANScript II RTase is an improved Moloney murine leukemia virus reverse transcriptase (M-MLV), and the improved new RTase has higher template affinity and lower RNase H activity, thus enabling it to read through complex secondary structure templates and reverse transcript long fragment cDNA in the synthesis process of the first strand of cDNA. The 5×TIANScript II RTase Buffer provided in the product has been carefully optimized, which not only ensures the high-efficiency enzyme activity of the new RTase, but also widens the input range of RNA template amount, making the reverse transcribed cDNA have better quality for subsequent experimental analysis.

Features
■ High enzyme activity and efficiency: High reverse transcriptase activity and good compatibility in subsequent experiments.
■ Wide substrate range: Suitable for all RNA, especially RNA templates with complex secondary structures.
■ Long RT length: The synthesis of the cDNA first strand can reach 12 kb.
■ Simple operation: Simply add the required components in one step without adding any reagent during the operation.

Applications
■ Synthesis of cDNA first strand.
■ Construction of cDNA library.
■ One-step RT-PCR.
■ RACE analysis.

TIANScriptⅡ RT Kit(Tiangen GKR107)

Publication

• Rhizoma Paridis saponins attenuate Gram??negative bacteria??induced inflammatory acne by binding to KEAP1 and modulating Nrf2 and MAPK pathwaysJournal of Cellular and Molecular Medicine2024-03-01 Read ArticleTIANScript II RT Kit (KR107??02; Tiangen; Shanghai; China) was used to synthesize cDNA. qPCR was performed
• Long non-coding RNA ACTA2-AS1 suppresses metastasis of papillary thyroid cancer via regulation of miR-4428/KLF9 axis.Clinical epigenetics2024-01-11 Read ArticleReverse transcription was performed using the TIANScript II RT Kit (Tiangen; Beijing; China) at 42 ??C.
• Long non-coding RNA ACTA2-AS1 suppresses metastasis of papillary thyroid cancer via regulation of miR-4428/KLF9 axisClinical Epigenetics2024-01-09 Read ArticleReverse transcription was performed using the TIANScript II RT Kit (Tiangen; Beijing; China) at 42 ??C.
• Molecular characteristics and pathogenicity of a novel chicken astrovirus variantVeterinary Research2023-12-08 Read Article.. USA) and then reverse transcribed into cDNA using the TIANScript II RT Kit (TIANGEN; Beijing; China).

TIANScriptⅡ RT Kit(Tiangen GKR107)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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