miRcute Plant miRNA Isolation Kit – Tiangen GDP504( Go2biotech.com – Distribution for Reagents and instruments )

miRcute Plant miRNA Isolation Kit – Tiangen GDP504

High-efficiency miRNA extraction kit specially targeting plant samples

Catalog number / packaging

 Manual

Storage
Store at room temperature (15-25°C).

Description
This kit can rapidly extract total RNA containing miRNA from plant tissues, especially plant tissues rich in polysaccharide, polyphenol or starch (such as cotton leaves, potato tubers, apples, peach tree leaves, etc.), and can simultaneously process a large number of different samples. The extracted total RNA has high purity and is free of protein and other impurities.

Features
■ The lysis buffer is specially optimized and equipped with sedimentation aid solution PLA, which is specially designed for the lysis of polysaccharide polyphenol plant samples with higher yield.
■ Column purification, free from impurity pollution, higher purity, suitable for downstream quantitative detection.
■ Comprehensive functions to extract total RNA in addition to small fragments.
■ The extraction can be completed within 1 h, improving the extraction efficiency.

Applications
It can be used in RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.

miRcute Plant miRNA Isolation Kit – Tiangen GDP504 Publication

• Protective effect of low??intensity pulsed ultrasound on immune checkpoint inhibitor-related myocarditis via fine-tuning CD4 + T-cell differentiationCancer Immunology, Immunotherapy2024-01-18 Read Article.. CD4 + T-cells was isolated using a RNeasy Isolation Kit (Tiangen Biotech; Beijing; China); and then the miRcute Plus
• Multi-omics analysis of small RNA, transcriptome, and degradome to identify putative miRNAs linked to MeJA regulated and oridonin biosynthesis in Isodon rubescens.International journal of biological macromolecules2023-12-30 Read ArticleAbstract: Isodon rubescens has garnered much attention due to its anti-tumor or anti-cancer properties.. However; little is known about the molecular mechanism of oridonin biosynthesis leveraging the regulatory network between small RNAs and mRNAs.. In this study; the regulatory networks of miRNAs and targets were examined by combining mRNA; miRNA; and degradome.
• Graphene enhances artemisinin production in the traditional medicinal plant Artemisia annua via dynamic physiological processes and miRNA regulation.Plant communications2023-11-02 Read Article.. miRNAs were isolated with the miRcute Plant miRNA Isolation Kit (Tiangen Biotech) according to the manufacturer??s protocol.
• Hydroxysafflor yellow A inhibits the hyperactivation of rat platelets by regulating the miR-9a-5p/SRC axis.Archives of biochemistry and biophysics2023-10-01 Read ArticleAbstract: Pathological platelet activation plays a vital role in the prevalence of cardiovascular diseases.. Hydroxysafflor yellow A (HSYA) has been shown to have significant anti-platelet aggregation and anti-activation effects; but its mechanism of action is unclear.. Our study showed that HSYA inhibited the expression of platelet surface glycoproteins II??/III ?? (GPII??/III ??) and thromboxane A2 (TXA2) during platelet activation and reduced platelet Ca2+ accumulation.
• Periodontal Ligament Stem Cell Exosomes Key to Regulate Periodontal Regeneration by miR-31-5p in Mice ModelInternational Journal of Nanomedicine2023-09-18 Read Article.. and qRT-PCR was conducted using MiRcute Enhanced miRNA Fluorescence Quantitative Detection Kit (Tiangen; Beijing; China) on a LightCycler

miRcute Plant miRNA Isolation Kit – Tiangen GDP504

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