miRcute Serum/Plasma miRNA Isolation Kit – Tiangen GDP503( Go2biotech.com – Distribution for Reagents and instruments )

miRcute Serum/Plasma miRNA Isolation Kit – Tiangen GDP503

High-efficiency miRNA extraction kit specifically targeting serum/plasma samples

Catalog number / packaging

Mat. NoRef. NoNo. of preps
4992865GDP50350 preps

 Manual

Storage
The extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

Description
miRcute Serum/Plasma miRNA Isolation Kit is a new generation of product specially developed for miRNA extraction from serum and plasma samples. The Buffer MZA in the kit has been well developed and improved, which has lysis capability and extraction sensitivity that are not available in common lysis solutions. The adsorption column in the kit adopts special silica matrix membrane filler, which greatly enhances its adsorption capability to RNA, especially small RNA(<200 nt). The extracted miRNA has better purity and higher quality, and is suitable for routine experiments such as downstream fluorescence quantification.

Features
■ High specificity: Specially designed for extracting miRNA in serum/plasma with optimized lysis buffer.
■ High efficiency: High quality miRNA can be obtained within 1 h with high extraction yield.
■ Ultra-pure: Column purification to avoid gDNA and salt contamination.

Applications
The extracted product can be used in routine experiments such as RT-qPCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning, etc.

miRcute Serum/Plasma miRNA Isolation Kit – Tiangen GDP503

Publication

  • Pulmonary tuberculosis biomarker miR-215-5p inhibits autophagosome-lysosome fusion in macrophages.Tuberculosis (Edinburgh, Scotland)2023-12-04 Read ArticleAbstract: The normal autophagy flux is beneficial for the rapid elimination of phagocytic pathogens by macrophages.. However; Mycobacterium tuberculosis interferes with the autophagy flux of macrophages to weaken their immune function and evade immune surveillance.. In this study; we found that miRNA-215-5p was downregulated in tuberculosis patients.
  • Sja-Let-7 Attenuates Carbon Tetrachloride-Induced Liver Fibrosis in a Mouse Model via Col1??2Biology2023-11-24 Read Article.. BALB/c mice were extracted using a miRcute Serum/Plasma miRNA Isolation Kit (TIANGEN; Shanghai; China) according to the manufacturer??s
  • The plasma derived exosomal miRNA-483-5p/502-5p serve as potential MCI biomarkers in aging.Experimental gerontology2024-01-10 Read Article.. in exosome were extracted using the miRcute miRNA Isolation Kit (TIANGEN; DP503) according to the manufacturer’s instruction
  • Parkinson’s disease patients combined with constipation tend to have higher serum expression of microRNA 29c, prominent neuropsychiatric disorders, possible RBD conversion, and a substandard quality of life.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology2023-08-11 Read ArticleAbstract: Introduction The symptom of constipation has been confirmed as an early diagnose criteria for Parkinson??s disease (PD).. Furthermore; evidences suggest that pathogenesis of PD initiates in gut; rather than brain.. If so; identifying biomarkers for constipation in PD might have potentials to assist early diagnosis and initial treatment.
  • microRNA-130b May Induce Cerebral Vasospasm after Subarachnoid Hemorrhage via Modulating Kruppel-like Factor 4Molecular and Cellular Biology2023-06-29 Read Article.. Tiangen) or a serum miRNA extraction and isolation kit (DP503; Tiangen) based on the instructions.

miRcute Serum/Plasma miRNA Isolation Kit – Tiangen GDP503

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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