miRcute Plus miRNA First-Strand cDNA Kit – Tiangen GKR211( Go2biotech.com – Distribution for Reagents and instruments )

miRcute Plus miRNA First-Strand cDNA Kit – Tiangen GKR211

Faster and more sensitive reagent for reverse transcription of miRNA by tailing method

Catalog number / packaging

 Manual

Storage
Store at -20°C

Description
The miRcute Plus miRNA cDNA First-Strand cDNA Kit adopts the A-tailing method to carry out reverse transcription of miRNA first strand cDNA. The kit adopts a two-component form, thus simplifying the experimental operation process and reducing the possibility of operation errors. miRNA RT Enzyme Mix in this product includes E.coli Poly(A) Polymerase, RTase and RNasin. The E. coli Poly (A) Polymerase not only has high A-tailing efficiency, but also can specifically recognize single-stranded miRNA, thus avoiding further reverse transcription reaction of miRNA precursors with double stranded structures. The RTase has been molecular modified to remove RNase H activity and increase the affinity of RNA template, thus enabling miRNA reverse transcription reaction to have higher efficiency and sensitivity. The 2×miRNA RT Reaction Buffer in this product contains all the raw materials and primers for miRNA A-tailing reaction and reverse transcription reaction. It is carefully optimized to ensure that the Poly(A) modification process and reverse transcription process at the 3’ end of miRNA can be efficiently carried out at the same time.

Features
■ Time-saving and labor-saving: A-tailing reaction and reverse transcription reaction is combined in one step, reducing the operation steps and saving half of the reaction time.
■ Strong specificity: Specific modification and reverse transcription of single-stranded miRNA to avoid the influence of miRNA precursor with secondary structure.
■ High sensitivity: All miRNA A-tailing products are used for further reverse transcription reaction, greatly improving the detection rate of low abundance miRNA.
■ Widely applicable: Reverse transcription reaction can be carried out on miRNA extracted from almost all materials. The input range of templates can reach 20 pg-2 μg (mass range) and 10 fM-100 pM (concentration range).

Applications
■ Real time quantitative study of miRNA by dye-based/probe-based method
Important Notes
■ This kit must be used together with miRcute Plus miRNA qPCR Kit (SYBR Green) (4992887/4992779)

Publication

• MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon ( Cucumis melo L.)Plants2024-04-02 Read Article.. strand cDNA was synthesized by the miRcute Plus miRNA first strand cDNA kit (TIANGEN; Beijing; China) and the expression levels
• The long noncoding RNA loc107053557 acts as a gga-miR-3530-5p sponge to suppress the replication of vvIBDV through regulating STAT1 expressionVirulence2024-03-25 Read Article.. that of miRNA was performed using miRcute miRNA First-strand cDNA Synthesis Kit (Tiangen; China).
• StMAPKK5 Positively Regulates Response to Drought and Salt Stress in PotatoInternational Journal of Molecular Sciences2024-03-25 Read ArticleThe total RNA extraction and cDNA first strand synthesis of potato cultivar ??Atlantic?? was performed using
• Precise large-fragment deletions in mammalian cells and mice generated by dCas9-controlled CRISPR/Cas3Science Advances2024-03-15 Read Article.. cDNA was synthesized using a cDNA first strand synthesis kit (TIANGEN; Beijing; China) and used for
• The long noncoding RNA LAL contributes to salinity tolerance by modulating LHCB1s?? expression in Medicago truncatulaCommunications Biology2024-03-08 Read Article.. cDNA was transcribed using miRcute Plus miRNA First-Strand cDNA kit (TIANGEN; China); and qRT-PCR was performed using miRcute

miRcute Plus miRNA First-Strand cDNA Kit – Tiangen GKR211

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