miRcute miRNA Isolation Kit – Tiangen GDP501 ( Go2biotech.com – Distribution for Reagents and instruments )

miRcute miRNA Isolation Kit – Tiangen GDP501

For high effective extraction of small fragment RNA at the range of 20-200 nt

Catalog number / packaging

 Manual

Storage
Store at room temperature (15-25℃ )
Buffer MZ should be stored at 2-8℃ away from light

Description
The miRcute miRNA Isolation Kit is a miRNA extraction and isolation kit based on the adsorption column method. It can efficiently extract small RNAs such as miRNA, siRNA, snRNA, etc., which are 20-200 nt in length, and can also extract total RNA. The efficient lysis buffer can easily lyse various tissues and cells. The adsorption column uses a special silicon matrix membrane packing, which greatly enhances its ability to adsorb RNA, especially small RNA (<200 nt).

Required Reagents
Ethanol, chloroform

Features
■ Efficient and accurate separation of small RNA fragments at the sizes of 20-200 nt.
■ Can be applied on small fragment RNA extraction as well as total RNA extraction.
■ Easy to operate, all operations can be completed in 1 hour.
■ High purity, the extracted RNA is free of DNA and protein contamination, facilitating the analysis of downstream experiments.

Applications
■ Northern Blot analysis.
■ Real-Time PCR.
■ Microarray analysis.

miRcute miRNA Isolation Kit – Tiangen GDP501

Publication

• The long noncoding RNA LAL contributes to salinity tolerance by modulating LHCB1s?? expression in Medicago truncatulaCommunications Biology2024-03-08 Read Article.. . siRNA cDNA was transcribed using miRcute Plus miRNA First-Strand cDNA kit (TIANGEN; China); and qRT-PCR was performed using
• Extracellular vesicles derived from HuMSCs alleviate daunorubicin-induced cardiac microvascular injury via miR-186-5p/PARP9/STAT1 signal pathwayRegenerative Therapy2024-02-03 Read ArticleTotal miRNA was extracted using the miRcute miRNA Isolation Kit (TIANGEN; China); and cDNA was synthesized employing miRcute
• IAA-miR164a-NAC100L1 module mediates symbiotic incompatibility of cucumber/pumpkin grafted seedlings through regulating callose depositionHorticulture Research2024-02-01 Read Article.. and RNA were isolated using the miRcute miRNA extraction kit (DP501; Tiangen; Beijing; China) and an RNAsimple Total
• Gestational Hypoxia Impaired Endothelial Nitric Oxide Synthesis Via miR??155??5p/NADPH Oxidase/Reactive Oxygen Species Axis in Male Offspring VesselsJournal of the American Heart Association2024-01-19 Read ArticleFor miRNA analysis; miRNAs from VECs were extracted using the miRcute miRNA isolation kit (Tiangen; Beijing; China).

and more..

miRcute miRNA Isolation Kit – Tiangen GDP501

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