1 kb DNA Ladder
Cat. no. 4992945/4992946
Storage: -30~-15°C for 2 years. For frequently use, it
can be stored at 2-8°C for 3 months.
Concentration: About 0.08 mg DNA/ml

Description
The 1kb DNA Ladder marker consists of nine doublestranded DNA bands that are suitable as molecular
weight standards for agarose gel electrophoresis.
Since this product is premixed with 1× Loading Buffer, it is convenient to load 3~6 μl of this product to agarose gel electrophoresis directly.
The ladder is composed of nine individual DNA fragments(in base pairs): 10000, 8000, 7000, 6000, 5000, 4000,3000, 2000, and 1000. It contains one intense band (5000bp) for easy orientation. To load 6 μl of the productwill provide 50 ng per DNA fragment with one intense fragment of 100 ng.

Buffer Contents
10 mM Tris-HCl (pH 8.4)
10 mM EDTA
0.02% Bromophenol Blue
5% Glycerol

Protocol

1. Directly load 3~6 μl of this product to the agarose gel(load 1 μl product per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).
2. Electrophoresis condition: 0.5~1.0% agarose gel, 4~10v/cm voltage.
3. DNA marker can be visualized using ethidium bromide under UV light.

Recommendations

1. Directly use, do not require heating.
2. For ideal results, please use new agarose gel and change electrophoresis buffer in time.

1 kb DNA Ladder

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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