DP114-TIANprep N96 Plasmid Kit

TIANprep N96 Plasmid Kit
For purification of molecular biology grade DNA

TIANprep N96 Plasmid Kit
Cat. no. 4992892/4992893
(N96 Plate)

Storage
TIANprep N96 Plasmid Kit can be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance
and quality. If any precipitate forms in the buffers, it should be dissolved
by warming the buffers at room temperature or 37°C water bath for 10
min before use. RNase A (10 mg/ml) can be stored for 15 months at room
temperature (15-30°C). After addition of RNase A, Buffer P1 is stable for 6
months at 2-8°C.

Introduction
TIANprep N96 Plasmid Kit uses spin plate which can specifically bind
plasmid DNA. The silica membrane within the spin plate is made of our
exclusive material which could specifically and efficiently bind plasmid
DNA. Proteins and other contaminant compounds can be properly
removed, and plasmid DNA that extracted by this kit is pure. No toxic
reagent like phenol and chloroform included in this kit.
Plasmid DNA prepared by TIANprep N96 Plasmid Kit is suitable for a variety
of molecular biological experiments including restriction enzyme digestion,
sequencing, library screening, ligation, transformation, etc.

Important Notes Before Using

1. Germiculture in N96 plate: Add 1.0-1.3 ml culture medium with
   antibiotic into each well of N96 Well Plate, pick single colony into each
   well and cover the plate. Grow cell culture at 37°C for 20-24 h with
   shaking (220-280 rpm).
2. Add the provided RNase A solution to Buffer P1 before use (please
   prepare in batches, add 1.25 ml RNase A concentration of 10 mg/ml
   per 125 ml P1, which may be used for 4-plate extraction reaction), mix,
   and store at 2-8°C.
3. Add 200 ml ethanol (96-100%) to 50 ml Buffer PW before using.
4. Check Buffer BL, P2 and P3 before use for salt precipitation. If necessary,
   dissolve the buffer by warming at 37°C water bath for several minutes.
   Immediately close the lid of Buffer P2 and P3 after use.
5. All centrifugation steps need to be carried out at room temperature (15-30°C).
6. The yield of plasmid DNA is related to cells concentration, bacteria
   strain and plasmid copy.
7. Using Buffer BL to treat spin plates could activate silica membrane and
   increase yield.
8. After treated with Buffer BL, use the Spin plate in the same day,
   otherwise lit may affect the purifying effect.

TIANRed user’s guide
TIANRed is a color indicator which is used to ensure the experimental
operation is correct. It has no negative effect on any downstream analysis
and no harm to human body. TIANRed is an optional reagent.
TIANRed should be added into Buffer P1 before use in the ratio of 1:200,
mix by reverting after the addition. Buffer P1 will turn red after the
addition of TIANRed. If the reaction turns purple after the addition of P2,
it indicates complete lysis. If the reaction turns yellow after the addition of
Buffer P3, it indicates complete renatuation.

DP114-TIANprep N96 Plasmid Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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