DP119-TIANprep N96 Magnetic Plasmid Kit

TIANprep N96 Magnetic Plasmid Kit

TIANprep N96 Magnetic Plasmid Kit
(N96 Plate)
Cat.no. 4992870

Storage
TIANprep N96 Magnetic Plasmid Kit can be stored dry at room temperature
(15-30°C) for up to 15 months without showing any reduction in
performance and quality. If any precipitate forms in the buffers, it should be
dissolved by warming the buffers to 37°C before use. RNase A (10 mg/ml)
can be stored for 15 months at room temperature (15-30°C). After adding
RNase A, Buffer P1 is stable for 6 months at 2-8°C.

Introduction
This kit adopts magnetic beads with special separating function and unique
buffer system, and can isolate and purify 2-20 µg high-quality plasmid DNA
from 1-1.5 ml bacterial solution. The unique embedded magnetic beads
have a strong affinity for plasmid DNA under certain conditions. When
the conditions change, the magnetic beads release the adsorbed plasmid
DNA, which can achieve the purpose of rapid separation and purification
of plasmid DNA, and can remove protein and other impurities to the
maximum extent, so as to ensure the purity of extracted plasmid DNA.
Plasmid DNA extracted by the kit can be used in various molecular biology
experiments, such as enzyme digestion, sequencing, library screening,
ligation, transformation, etc.

Important Notes Before Using

1. Please add RNaseA in Buffer P1 before use (the addition ratio is
   P1:RNaseA = 100:1), mixed evenly, and stored at 2-8°C.
2. Please add the corresponding volume of 96-100% ethanol to Buffer PW
   according to the label on the bottle before the using for the first time.
3. Before use, check whether Buffer P2 and Buffer P3 are turbid. If yes,
   incubate them in a 37°C water bath for a few minutes to restore
   clarification. Tighten the cap of Buffer P2 and Buffer P3 immediately
   after use.
4. All centrifugation steps shall be conducted at room temperature.
5. The amount of plasmid extracted is related to the culture concentration
   of bacteria, host bacteria, plasmid copy number and other factors.
6. The magnetic beads suspension should be fully mixed before use.

DP119-TIANprep N96 Magnetic Plasmid Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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