DP205-TIANquick Maxi Purification Kit

TIANquick Maxi Purification Kit
For purification of PCR products, 100 bp to 10 kb

TIANquick Maxi Purification Kit
(Spin Column)
Cat. no. 4992894

Storage
TIANquick Maxi Purification Kit can be stored dry at room temperature (15-30°C) for up to 15 months without showing any reduction in performance
and quality. If any precipitate forms in the buffers, it should be dissolved by
warming the buffers at 37°C for 10 min before use.

Introduction
TIANquick Maxi DNA Purification Kit applies unique silica-membrane
technology to purify 100 bp-10 kb DNA fragments with over 80% recovery
yield from enzymatic reactions and PCR products, meantime remove
contaminants of protein, other organic compound, salts and primers, etc.
The Spin Column could bind 20 μg DNA per column.
Purified DNA by the kit can be directly used in applications such as
restriction enzyme digestion, PCR, sequencing, library screening, ligation,
transformation, etc.

Important Notes Please read the notes before using this kit.

1. This kit is applied in purification of all DNA fragments exist in solution
   non-selectively (fragments <50 bp could be removed). If need to purify
   specific DNA fragments, meantime remove all other fragments, please
   choose gel purification kits.
2. Adding volume of Buffer EB should be determined by DNA volume before
   purification: for 1-5 μg DNA, choose Spin Column CB1, add 20-50 μl Buffer
   EB; for 5-20 μg DNA, choose Spin Column CB2, add 30-100 μl Buffer EB;
   for 20-30 μg DNA, choose Spin Column CB3, add 50-300 μl Buffer EB.
3. The recovery efficiency is related to starting DNA volume and elution
   volume. Insufficient starting DNA or elution volume reduces the
   recovery efficiency significantly.
4. Increase absorption and elution time could improve recovery efficiency
   for <100 bp and >10 kb DNA fragment.
5. Buffer BL can improve the absorption capability and stability of the
   silica membrane, eliminate the effect of bad circumstance such as
   high temperature or humid on silica membrane performance. Check
   Buffer BL before use for salt precipitation. Redissolve any precipitate by
   warming at 37°C for several minutes.
6. After treated with Buffer BL, use the Spin Column as soon as possible (in
   one day).

DP205-TIANquick Maxi Purification Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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