morgan@go2biotech.comDP213-TIANquick N96 Purification Kit
TIANquick N96 Purification Kit
TIANquick N96 Purification Kit
Cat. no. 4992871/4992872
(N96 Plate)
Storage
TIANquick N96 Purification Kit can be stored under dry conditions at room
temperature (15-30°C) for up to 15 months without showing any reduction
in performance and quality. If any precipitate forms in the buffers, it should
be dissolved by warming the buffers at 37°C for 10 min before use.
Introduction
The kit uses a unique adsorption column to purify DNA fragments in
reaction solutions such as enzyme digestion and PCR. It can remove
contaminants such as protein, other organic compound, salts and primers.
The fragments purified range from 100 bp to 30 kb with over 80% of
recovery efficiency. The maximum amount of DNA that can be adsorbed
per well is 5 μg.
DNA recovered with this kit can be used for various operations,
including enzyme digestion, PCR, sequencing, library screening, ligation ,
transformation, etc.
Product features
Fast: The whole operation process only takes a few tens of minutes, saving
time to the greatest extent.
Efficient: The unique adsorption plate and the specially prepared buffers
allow maximum recovery of DNA with a high purity.
Important Notes Please read the notes before using this kit.
- This kit is suitable for non-selective recovery of all DNA fragments in the
solution (small fragments below 30 bp can be removed). If necessary to
recover specific fragments while removing other fragments of different
sizes, please select the gel purification kit. - The recovery yield is related to the initial amount of DNA and elution
volume. The lower the initial amount of DNA, the smaller the elution
volume, and the lower the recovery yield. The elution volume should
preferably not be less than 60 μl per well. - Before each use, mix 200 ml ethanol (96-100%) with 50 ml Buffer PW.
- Before use, check whether the Buffer BL is turbid. If there is turbid
phenomenon, incubate in a 37°C water bath for a few minutes. - Treating the N96 Plate CB2 with the Buffer BL before use can active the
silicon matrix membrane and improve the yield. - The N96 Plate CB2 treated with Buffer BL is best used on the same day,
and the effect will be affected if it is placed for too long.
Vacuum protocol
- Connect the negative pressure vacuum device correctly and place the
N96 Plate CB2 on the negative pressure vacuum device. Add 200 μl of
Buffer BL to each well of N96 Plate CB2, turn on and adjust the negative
pressure vacuum, and absorb all the solution in the plate. - Adding 3 times the volume of Buffer PB to PCR, enzyme digestion, enzyme
labeling or sequencing reaction solution; After mixing evenly, transfer to
balanced N96 Plate CB2, place at room temperature for 2 min, turn on
and adjust negative pressure to absorb all the solution in the plate. - 200 μl of Buffer PW was added to each well of N96 Plate CB2 to absorb
all that solution in the plate. - Repeat Step 3.
- The N96 Plate CB2 was sucked at the maximum negative pressure for 10 min.
Notes: The residue of ethanol will affect the subsequent enzyme
reaction (enzyme digestion, PCR, etc.) . - The N96 Plate CB2 is slapped 6 times on the absorbent paper with the
guide pipe facing down. - The N96 Plate CB2 is placed on the N96 Well Plate, and 80-100 μl ddH2
O
(pH ≥ 7.5) or Buffer TB is added, stand at room temperature for 5 min.
Centrifuge at 3,600 rpm for 10 min and collect the DNA solution. - The N96 Well Plate was covered with a new plate cover, and store the
DNA solution at -20°C for standby.
DP213-TIANquick N96 Purification Kit
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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.
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E-mail: maggie@go2biotech.com / morgan@go2biotech.com
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