EM101-Methylation-specific PCR (MSP) Kit

Methylation-specific
PCR (MSP) Kit
Cat. No. 4992759
Storage: It can be stored for 12 months at -30~-15°C.
Concentration: 2.5 U/μl

Introduction
Epigenetics is a branch of genetics that studies the heritable changes of gene expression and regulation
without changing the nucleotide sequence of genes.
There are many epigenetic phenomena, such as RNA mediated gene silencing and histone modification. One of the major epigenetic mechanisms in higher eukaryotes is DNA methylation.
This product is a kit specially developed for customers who study the methylation characteristics of genomic DNA by PCR. The kit has simple components, including MSP DNA Polymerase, 10×MSP PCR Buffer and dNTPs.
Among them, MSP DNA Polymerase is a thermostable polymerase modified with antibody, and 10×MSP PCR Buffer is a PCR buffer optimized especially for MSP reaction. The product has the advantages of rapidness, simplicity, high sensitivity, strong specificity, good stability, etc. It is suitable to be used together with DNA Bisulfite Conversion Kit (Cat.no. 4992447).

10×MSP PCR Buffer:
500 mM Tris-HCl (pH8.8)
200 mM KCl
15 mM MgCl2
Other stabilizers and enhancers

Quality Control
Purity up to standard by SDS-PAGE assay; No activity of exogenous nuclease is detected; Single copy gene in human genome could be amplified effectively;
No significant activity change when stored at room temperature (15-30°C) for one week.

Description
The product is convenient and quick to use, and can avoid contamination during PCR operation. When in use, simply add a proper amount of MSP DNA Polymerase, 10×MSP PCR Buffer and dNTPs, then add templates and primers, and finally top up the volume with ddH2O to make the 1×MSP PCR Buffer concentration for PCR reaction.

Applications
This product is suitable for analyzing the methylation characteristics of genomic DNA by methylation specific PCR (MSP) method.

Example
Note: The following example is for reference only. The actual reaction conditions vary according to different reaction conditions such as templates, primers, etc.
The optimal reaction conditions should be set according to the actual situation

EM101-Methylation-specific PCR (MSP) Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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