EndoFree Maxi Plasmid Kit V2

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Purification of endotoxin-free transfection grade plasmid DNA specific for sensitive cells

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• StorageRoom temperature (15-25℃ )
• DescriptionThe EndoFree Maxi Plasmid Kit V2 adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. The special Buffer ER and the Filtration CS1 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 1 hour.Recommended bacteria culture medium amount: For high-copy plasmid, 100 ml bacteria medium is recommended, which can yield up to 500-1500 μg plasmid. For low-copy plasmid, 200 ml of bacteria medium is recommended to achieve the yield of around 50-300 μg.
• Required ReagentsEthanol, isopropanol
• Features■ High purity: The unique endotoxin precipitation technology is adopted to specifically remove endotoxin.■ Easy to operate: The adsorption column technology is used to specifically adsorb plasmid DNA, which makes the operation easier.■ High-efficiency transfection: Suitable for the transfection of most cell lines including endotoxin-sensitive cells.■ Wide range of applications: The purified plasmid can be applied in the transfection of animal and plant cells as well as molecular biology experiments.
• ApplicationsThe plasmid DNA extracted using this kit can be used for various regular operations, including restriction enzyme digestion, PCR, sequencing, ligation, transformation and transfection of various cells.
• Experimental Procedure

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• Spatially resolved mapping of proteome turnover dynamics with subcellular precisionNature Communications2023-11-08 Read ArticlePlasmids were extracted from E. coli by EndoFree Mini Plasmid Kit II (Tiangen).
• In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapy.Cell death & disease2023-09-25 Read Article.. later; plasmids were extracted using an EndoFree Maxi Plasmid Kit V2 (Tiangen; DP120; Beijing; China) according to the
• In vivo self-assembly and delivery of VEGFR2 siRNA-encapsulated small extracellular vesicles for lung metastatic osteosarcoma therapyCell Death & Disease2023-09-22 Read Article.. later; plasmids were extracted using an EndoFree Maxi Plasmid Kit V2 (Tiangen; DP120; Beijing; China) according to the

EndoFree Maxi Plasmid Kit V2

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