EndoFree Mini Plasmid Kit II

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For purification of endotoxin-free transfection grade plasmid DNA

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• StorageRoom temperature (15-25℃ )
• DescriptionThe EndoFree Mini Plasmid Kit II adopts a unique silica membrane adsorption technology to efficiently and specifically bind plasmid DNA. The special endotoxin-removing system can effectively remove impurities such as endotoxin and proteins. The whole extraction process takes only 1 hour, which is convenient and quick. The plasmid extraction yield is related to the plasmid copy number, host bacteria type and the culture conditions. Up to 70 μg of plasmid DNA can be extracted from 5-15 ml of the overnight cultured bacteria culture medium.
• Required ReagentsEthanol, isopropanol
• Features■ Rapid and high yield: 5-70 μg plasmid DNA can be extracted in around 1 hour.■ High-purity: The special endotoxin removal system combined with Spin Column CP4 can specifically adsorb nucleic acid and remove endotoxin to the minimal level.■ Wide range of applications: Suitable for routine experiments such as restriction endonuclease digestion, PCR, sequencing, ligation, transformation, as well as the high-precision experiments such as gene therapy, cell microinjection, gene silencing, transcription, etc.
• Plasmid Yield

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• RGD-p21Ras-scFv expressed prokaryotically on a pilot scale inhibits ras-driven colorectal cancer growth by blocking p21Ras-GTPBMC Cancer2024-01-12 Read Article.. standard experimental step using the EndoFree Mini Plasmid Kit II (Cat. #DP118-02) from TIANGEN BIOTECH (Beijing) Co.; Ltd.; and stored
• Reversible phosphorylation of a lectin-receptor-like kinase controls xylem immunity.Cell host & microbe2023-12-16 Read ArticleAbstract: Article. kinase controls xylem immunity. d The lectin-receptor-like kinase LORE regulates ROS production in root xylem d Phosphorylation of LORE is important for xylem immunity d LORE-associated phosphatase LOPP dephosphorylates LORE to attenuate xylem immunity d LOPP knockout enhances resistance against bacterial wilt in Arabidopsis and tomatoes Wang et al.; 2023; Cell Host & Microbe 31; 2051??2066 December 13; 2023 ?? 2023 Elsevier Inc. https://doi.org/10.1016/j.chom.2023.10.017 Authors Ran Wang; Chenying Li; Zhiyi Jia; …; Zeng Tao; Fucheng Lin; Yan Liang Correspondence fuchenglin@zju.edu.cn (F.L.); yanliang@zju.edu.cn (Y.L.)
• Orally-Delivered, Cytokine-Engineered Extracellular Vesicles for Targeted Treatment of Inflammatory Bowel Disease.Small (Weinheim an der Bergstrasse, Germany)2023-12-16 Read ArticleAbstract: The use of orally-administered therapeutic proteins for treatment of inflammatory bowel disease (IBD) has been limited due to the harsh gastrointestinal environment and low bioavailability that affects delivery to diseased sites.. Here; a nested delivery system; termed Gal-IL10-EVs (C/A) that protects interleukin 10 (IL-10) from degradation in the stomach and enables targeted delivery of IL-10 to inflammatory macrophages infiltrating the colonic lamina propria; is reported.. Extracellular vesicles (EVs) carrying IL-10 are designed to be secreted from genetically engineered mammalian cells by a plasmid system; and EVs are subsequently modified with galactose; endowing the targeted IL-10 delivery to inflammatory macrophages.
• Regulation of epithelial cell differentiation by the Ubiquitous expressed transcript isoform 1 in ulcerative colitis.Journal of gastroenterology and hepatology2023-11-27 Read ArticleAll plasmids were isolated using endotoxin-free plasmid extraction kit (Tiangen; DP118-02) and confirmed through DNA sequencing.
• CHD1L knockdown suppresses bladder cancer cell growth in vitroEurope PMC2023-10-28 Read ArticleThe selected high purity plasmids containing interference sequence were extracted by EndoFree midi Plasmid Kit (Cat. #DP118-2; TIANGEN).

EndoFree Mini Plasmid Kit II