EP101-Pfu DNA Polymerase

PfuDNA Polymerase
Cat. no. 4992760
Storage: -30~-15°C for two years.
Concentration: 2.5 U/µl


Introduction
PfuDNA Polymerase is a themostable enzyme with a molecular weight of approximately 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5′-3′ direction in the presence of magnesium and it also exhibits 3′-5′ exonuclease (proofreading) activity,
resulting in blunt-ended PCR products without 3′ -dA overhanges. PfuDNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity DNA synthesis.
Unit Definition One unit of PfuDNA Polymerase is defined as the amount that incorporates 10 nmol of dNTPs into acidinsoluble material within 30 min at 74°C with activated salmon sperm DNA as the template/primer.


Storage Buffer
50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT,
Stabilizers, 50% Glycerol.


Applications
High fidelity DNA amplification, such as DNA sequencing,gene expression & cloning, gene site-directed
mutagenesis, SNP, end repairing, etc.

10× PfuBuffer
200 mM Tris-HCl (pH 8.8), 100 mM KCl, 100 mM(NH4)
2SO4 , 20 mM MgSO4, other components.


Quality Control
Purity up to standard by SDS-PAGE assay. No exogenous
nuclease activity is detected. Single copy gene in human
genome could be amplified effectively. No significant
activity change when stored at room temperature (15-30°C) for one week.


Note

  1. The extension of PfuDNA Polymerase is about 500-1000 bp/min normally. Because Pfu DNA Polymerasewith 3’-5’ exonulease activity may degrade the primers, it should be added later than other components, and immediately go to PCR step.
  2. As PfuDNA Polymerase has superior thermostability compared to TaqDNA Polymerase, for high-GC
    templates, temperature of denaturation can be increased to 98°C, which doesn’t have any influence
    on PfuDNA Polymerase’s activity.

EP101-Pfu DNA Polymerase

All trademarks or registered trademarks appearing on this website are the property of their respective owners.

This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

Need more info ? Contact us anytime. We’re here: Go2biotech
E-mail: maggie@go2biotech.com / morgan@go2biotech.com
Telephone:+86 755 8399 5017