FastKing gDNA Dispelling RT SuperMix-GKR118

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gDNA removal and reverse transcription in 18 minutes

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• Packing Size20 μl×25 rxn
• StorageStore at -20℃.
• DescriptionThis product is a highly efficient, stable, fast reverse transcription premix that can remove genomic DNA contamination. 5×FastKing-RT SuperMix contains not only all reagents required for reverse transcription reaction in RT- PCR, but also thermosensitive gDNAse for efficient removal of genomic DNA. FastKing RT Enzyme is a new type of reverse transcriptase by molecular modification. It has outstanding performance in the aspects of RNA template with high GC content, complex secondary structure and stress resistance. The thermosensitive gDNase has fast effect, high efficiency and short reaction time, and will not affect cDNA quality after treating residual genomic DNA. The combination of the two enzymes enable the depletion of the residual genome and the reverse transcription reaction simultaneously, thus greatly facilitating the reverse transcription reaction.
• Features■ Fast: One-step to complete genome removal and efficient reverse transcription within 18 minutes by only adding templates.■ High efficiency: The reverse transcriptase is modified with a hydrophobic motif, with RT efficiency exceeding 95%.■ Sensitive: As low as 1 ng templates can be accurately identified.■ Simple and easy: The exclusive thermosensitive DNase has fast effect, high efficiency with shorter reaction time, and will not affect cDNA.
• ApplicationsThe reverse transcribed cDNA can be used in conventional PCR, Real time PCR, cDNA library construction, SAGE (Serial Analysis of Gene Expression), primer extension and other conventional experiments.

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• Cerebral venous congestion alters CNS homeostatic plasticity, evoking tinnitus-like behaviorCell & Bioscience2024-04-09 Read ArticleThen; cDNA was synthesized using FastKing gDNA Dispelling RT SuperMix (TIANGEN).
• Regulation of quorum sensing activities by the stringent response gene rsh in sphingomonads is species-specific and culture condition dependentFrontiers in Microbiology2024-04-04 Read Article.. manufacturer??s instructions. cDNA was synthesized using FastKing gDNA Dispelling RT SuperMix (Tiangen Biotech; Beijing; China) and was used as
• Effects of dietary cecropin on growth performance, diarrhea rate and intestinal health of nursery Hainan pigsFrontiers in Microbiology2024-04-03 Read Article.. reverse transcribed into cDNA using FastKing gDNA Dispelling RT SuperMix (TIANGEN; Beijing) according to the manufacturer??s
• 17??-Estradiol protects female rats from bilateral oophorectomy-induced nonalcoholic fatty liver disease induced by improving linoleic acid metabolism alteration and gut microbiota disturbanceHeliyon2024-04-02 Read Article.. transcription was performed by FastKing gDNA Dispelling RT SuperMix (KR118; TIANGEN Biotech). qRT??PCR was conducted by TransStart??
• Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca 2+ overload via IP3R-GRP75-VDAC1 pathway.Ecotoxicology and environmental safety2024-03-29 Read Article.. 20 ??L reaction volume; with FastKing gDNA Dispelling RT SuperMix (TIANGEN Biotech; Beijing; China) as a reagent.

FastKing gDNA Dispelling RT SuperMix

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