FastKing One Step RT-qPCR Kit (Probe)-GFP314

.. 185 / Publication

More sensitive and efficient one-step reverse and transcription probe fluorescence quantitative reagents

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• DescriptionThis product is a special reagent for one-step RT-qPCR using probe method (TaqMan, Molecular Beacon, etc.). The One Step RT-qPCR reaction can be continuously carried out in the same reaction tube using the product. The operation is simple. The cross contamination between samples can be avoided and the detection sensitivity is improved. The kit adopts a premix of TIANGEN novel reverse transcriptase (King RTase), novel antibody modified hot start Taq DNA polymerase and RNase Inhibitor, and has stronger RNA affinity and thermal stability, and higher amplification efficiency and specificity. In addition, the 2×FastKing One Step Probe RT-qPCR MasterMix in this product is a new type of reaction system specially optimized for the above two key enzymes. It contains necessary ionic components, dNTPs, PCR stabilizers and enhancers, which can ensure the best performance of King RTase and the new type of hot start Taq DNA polymerase in the whole one-step reaction process.
• StorageStore at -20℃.
• Features■No cross-contamination: Reverse transcription and fluorescence quantification are completed in one step to avoid cross contamination.■ Sensitive: Templates as low as 1 ng can be accurately identified, especially suitable for templates with low abundance.■ Stress resistance: Can be applied on complex templates, perfect against foreign interference, suitable for various templates■ Simple operation: Optimize product composition and improve experimental efficiency.
• ApplicationsThe kit is suitable for probe one-step Real-Time RT-PCR, it can accurately and simply perform mRNA expression analysis. It is especially suitable for detection of trace RNA on various real time PCR instruments.

Publication

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• Profiles of Intestinal Flora in Breastfed Obese Children and Selecting Functional Strains Against Obesity.Molecular nutrition & food research2024-03-01 Read ArticleAbstract: Scope: Breast milk has the potential to prevent childhood obesity by providing probiotics; but there are still instances of obesity in breastfed children.. Methods and Results: This study investigates the difference in intestinal flora structure between breastfed children with obesity (OB-BF) and normal-weight breastfed children (N-BF).. Building upon this foundation; it employs both cell and mouse models to identify an antiobesity strain within the fecal matter of N-BF children and explore its underlying mechanisms.
• The metabolism-related lncRNA signature predicts the prognosis of breast cancer patientsScientific Reports2024-02-12 Read ArticleThe FastKing One Step Kit (TIANGEN Biotech; Beijing; China) was utilized to perform qRT-PCR following the manufacturer’s protocol.
• Small Extracellular Vesicles Released from miR-211-5p-Overexpressed Bone Marrow Mesenchymal Stem Cells Ameliorate Spinal Cord Injuries in RatseNeuro2024-02-09 Read Article.. was performed using a FastKing One Step RT-qPCR Kit (SYBR Green) purchased from Tiangen Biotech following the directions. ??-Actin
• Role of myeloid-derived suppressor cells in chronic brucellosisFrontiers in Cellular and Infection Microbiology2024-01-30 Read ArticleRT-qPCR was performed with SYBR One Step RT-qPCR kit (FastKing Real Time One Step RT-qPCR; Tiangen; China) using ??-actin gene as a control for normalization of the data.
• Analysis of Polyphenol Extract from Hazel Leaf and Ameliorative Efficacy and Mechanism against Hyperuricemia Zebrafish Model via Network Pharmacology and Molecular Docking.Molecules (Basel, Switzerland)2024-01-24 Read Article.. cycles were performed using FastKing One Step Reverse Transcription Fluorescence Quantification Kit (SYBR Green) (FP313; TIANGEN; Beijing; China)

FastKing One Step RT-qPCR Kit (Probe)

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