FP215-SuperReal PreMix Color (SYBR Green)

SuperReal PreMix Color (SYBR Green)
——Considerate and specific dye-based fluorescence quantitative reagent

SuperReal PreMix Color (SYBR Green)
Cat. No. 4992777/4992876/4992902

Storage
The product can be stored at -30~-15°C for 1 year.After receiving this
product, please immediately place it at -30~-15°Cand keep it away
from light. When taking out from -30~-15°Cfor use, dissolve the frozen
2× SuperReal Color PreMix and 50× ROX Reference Dye, then gently
inverse the tube to mix them evenly. Please use them after the solution is
completely uniform. If it is not used after thawing, it must be thoroughly
mixed and then frozen again (salt will be delaminated during thawing; if
it is not mixed before freezing, the precipitation of salt crystals will cause
damage to enzymes). If it needs to be used frequently for a period of
time, it can be stored at 2 – 8°C for 3 months. Avoid repeated freezing and
thawing.

Introduction
This product is a special reagent for real-time PCR using SYBR Green I
chimeric fluorescence. The reaction reagent is a 2× concentration PreMix
with SYBR Green I at the optimum concentration required for real-time
PCR. The indicator is additionally added to the product, which is convenient
for adding a large number of samples and reduces the probability of
misoperation.
SuperReal Color PreMix adopts a unique two-component HotStart DNA
polymerase (chemically modified HotStart TaqDNA polymerase and
antibody modified Anti TaqDNA polymerase). Cooperating with a carefully
optimized buffer system, this product has high amplification efficiency
and high specificity and wider credible range. The improved reagent
has prominent advantage in amplification specificity after composition
adjustment

Features

1. SuperReal Color PreMix uses a unique two-component HotStart DNA
   polymerase (chemically modified HotStart TaqDNA polymerase and
   antibody modified Anti TaqDNA polymerase) to form an enzyme
   activity automatic regulation system, which is characterized by high
   amplification efficiency, high amplification specificity and wide credible
   range in cooperation with a carefully optimized buffer system.
2. The Buffer system of this product balances the ratio of K+and NH4+
   , and specially adds the original H-Bond factor, which can coordinate to adjust
   the hydrogen bond force in the reaction system, so that the annealing
   conditions of primers and templates are more rigorous, the specificity
   of the reaction is enhanced, and the repeatability is better.
3. SuperReal Color PreMix is pre-mixed with SYBR Green I. When preparing
   PCR reaction solution, the Real Time PCR reaction can be carried out by
   simply adding templates, primers and RNase-Free ultrapure water. The
   operation is simple and convenient.
4. This product comes with ROX Reference Dye, which is used to eliminate
   the signal background and to correct the fluorescence signal error
   produced between holes. The ROX Reference Dye is convenient for
   users to select the corresponding concentration for different types of
   fluorescence quantitative PCR instruments.
5. The addition of color indicator makes sample addition easier and
   effectively reduces the probability of misoperation.

FP215-SuperReal PreMix Color (SYBR Green)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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