FP402-lnRcute lncRNA qPCR Kit(SYBR Green)

lnRcute lncRNA qPCR Kit (SYBR Green)
——Sensitive and stress-resistant special fluorescent quantitative reagent for lncRNA

lnRcute lncRNA qPCR Kit
(SYBR Green)
Cat. no. 4992778/4992886

Storage
Please store the product at -30~-15°Cimmediately after receiving it.
When taking out from -30~-15°Cfor use, dissolve the frozen 2× lnR lncRNA
PreMix and ROX Reference Dye, and then mix them gently by inverting up
and down until the solution is completely uniform before use. If it is not
used after thawing, it must be thoroughly mixed and then frozen again
(salt will be delaminated during thawing; if it is not mixed before freezing,
the precipitation of salt crystals will cause damage to enzymes). If it needs
to be used frequently for a period of time, it can be stored at 2-8°C for 3
months. Avoid repeated freezing and thawing. This product can be stored
at -30~-15°Cfor 1 year.

Introduction
The kit adopts the principle of SYBR Green I chimeric fluorescence to carry
out fluorescence quantitative detection of lncRNA. The kit contains all
reagents for quantitative detection of lncRNA, including 2× lnR lncRNA
PreMix, 50× ROX Reference Dye and RNase-Free ddH2
O. The target lncRNA
can be rapidly and specifically quantitatified by this kit.
Compared with mRNA, lncRNA has the characteristics of low abundance, large
difference in GC content and more complex secondary structure. Traditional
quantitative reagents are difficult to achieve ideal effects on lncRNA. The 2×
lnR lncRNA PreMix in this kit is a new generation of pre-mixed fluorescent
quantitative PCR detection reagent specially developed for the quantitative
detection of lncRNA. The DNA Polymerase in this kit is an antibody modified
hotstart enzyme, and matching with a specially optimized buffer system, it can
ensure that the product has higher detection sensitivity while ensuring higher
reaction specificity. In addition, the addition of H-competitor factor and EP
component in the buffer system ensures a wide range of sample universality,
and has very good amplification applicability for templates with different
GC contents, templates with complex advanced structures, templates with
more PCR inhibitor residues and long fragment templates, etc. It is especially
suitable for quantitative detection of lncRNA with relatively complex advanced
structures and relatively low overall abundance. In addition, the optimized
premix can shorten the reaction time of Real-Time PCR and is suitable for
standard or rapid quantitative PCR reactions.

Features

1. Easy operation:SYBR Green I is pre-mixed in 2× lnR lncRNA PreMix.
   When preparing PCR reaction solution, simply add template, primers and
   sterilized distilled water to carry out fast Real-Time PCR reaction, which is
   simple and convenient to operate.
2. Good universality: The product contains H-Competitor factor, which
   can compete for hydrogen bonds and enhance the opening strength of
   double strands, so that the product has wide sample universality, and has
   superior applicability to templates with complex advanced structures,
   long fragment amplification, etc.
3. Excellent performance:The special fast PCR Buffer system of this product
   contains a unique PCR stabilizing factor-EP, which can effectively protect
   the enzyme activity and resist the interference of various PCR inhibitors,
   ensuring that 2× lnR lncRNA PreMix has the characteristics of high
   amplification efficiency, high amplification specificity, high amplification
   sensitivity and wide credible range.
4. Accurate quantification: The ROX Reference Dye supplied in this product
   can be used to eliminate the signal background and to correct the error
   of fluorescence signal generated between wells, and is convenient
   for users to select corresponding concentrations for different types of
   fluorescence quantitative PCR instruments.
5. Good stability:2× lnR lncRNA PreMix is packed in colorless transparent
   tube. After detection, light will not affect the quantitative results of the
   system.

Precautions Please carefully read these precausions before use.

1. If the reagents are not mixed evenly, the reaction performance will be
   decreased. When using, please mix it by gently inverting up and down.
   Please do not mix it evenly with vortex, try to avoid foam, and use it
   after instantaneous centrifugation.
2. Primer purity has a great influence on reaction specificity, so it is
   recommended to use primers purified above PAGE level.
3. In the 20 µl reaction system, the amount of cDNA template used is
   generally less than 100 ng, and the amount of genomic DNA template
   is generally less than 50 ng. When the reverse transcription product is
   used as the template, the amount used should not exceed 10% of the
   final volume of PCR system.

FP402-lnRcute lncRNA qPCR Kit(SYBR Green)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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