GDP219-TIANgel Purification Kit

TIANgel Purification Kit
Room temperature gel dissolution, fast and high efficient gel and PCR product recovery

TIANgel Purification Kit
Cat. no. GDP219
(Spin Column)

Storage
TIANgel Purification Kit should be stored dry at room temperature (15-30°C)
for up to 15 months without showing any reduction in performance and
quality. If any precipitate forms in the buffers, it should be dissolved by
warming the buffers at 37°C for 10 min before use.

Introduction
TIANgel Purification Kit uses a silicon matrix material that can efficiently
and specifically bind DNA and a unique, and this kit can recover DNA
fragments from TAE or TBE agarose gel while removing impurities such as
proteins, other organic compounds, inorganic salt ions and oligonucleotide
primer, etc. The purified DNA fragment range is 100 bp-15 kb with up to
80% of recovery efficiency. Each spin-column can adsorb 10 μg of DNA.
DNA recovered with this kit can be used for various operations,
including enzyme digestion, PCR, sequencing, library screening, ligation,
transformation, etc.

Features
Fast:The entire operating process is very fast and convenient and can be
finished within 10 minutes.
Diversity: This kit is capable of recovering single-strand and double-strand
DNA segments, as well as ring-shaped plasmid DNA.
Efficient: The unique spin-column and the specially buffers allow maximum
recovery of DNA with a high purity.

Important Notes Please read the notes before using this kit.

1. Use fresh buffer when performing electrophoresis to avoid impacts on
   the electrophoresis and the recovery efficiency.
2. It is better to use TAE buffer if there is high demand for following
   experiment.
3. All centrifugation steps are performed using a benchtop centrifuge at
   room temperature.
4. When cutting the gel, the time of ultraviolet radiation shall be kept to
   the shortest to prevent damage to DNA.
5. Test the pH value after the gel is sufficiently dissolved if the recovery
   rate is low. If the pH value is greater than 7.5, you may add 10-30 µl
   3 M CH3
   COONa (pH5.2) into the gel solution containing DNA until the
   solution pH adjusted to 5-7.
6. The recovery efficiency is related to the initial amount of DNA and the
   elution volume. The recovery efficiency will be low if the initial DNA
   amount and the elution volume are small.

Reagents need to be prepared by Customer
96-100% ethanol,3 M CH3 COONa (pH5.2)

GDP219-TIANgel Purification Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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