MD103-DNA Marker III

DNA Marker III
Cat. no. 4992939/4992940
Storage: -30~-15°C for 2 years. For frequently use, it can be stored at 2-8°C for 3 months.
Concentration: About 0.06 mg DNA/ml

Description
DNA Marker III consists of seven double-stranded DNA bands that are suitable as molecular weight standards for agarose gel electrophoresis.
The product is ready-to-use; it is premixed with 1×Loading Buffer for direct loading 3~6 μl on agarose gel electrophoresis.
The ladder is composed of seven individual DNA fragments (in base pairs): 4500, 3000, 2000, 1200, 800,
500, and 200. It contains one intense band (1200 bp) for easy orientation. To load 6 μl of the product will provide 50 ng per DNA fragment with one intense fragment of 100 ng.

Buffer Contents
10 mM Tris-HCl (pH 8.4)
10 mM EDTA
0.02% Bromophenol Blue
5% Glycerol

Protocol

1. Directly load 3~6 μl of this product on the agarose gel(load 1 μl per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).
2. Electrophoresis condition: 0.7~2% agarose gel, 4~10 v/cm voltage.
3. DNA marker can be visualized using ethidium bromide under UV light.

Recommendations

1. Directly use, do not heat before loading.
2. Please use new agarose gel and change electrophoresis buffer in time, so as not to affect the electrophoresis results.

MD103-DNA Marker III

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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