MD110-D15000

D15000
Cat. no. 4992943/4992944
Storage: -30~-15°C for 2 years. For frequently use, it can
be stored at 2-8°C for 3 months.
Concentration: About 0.06 mg DNA/ml

Description
Marker D15000 consists of seven double-stranded DNA bands are suitable as molecular weight standards for agarose gel electrophoresis.
The product is ready-to-use; it is premixed with 1×Loading Buffer for direct loading 3~6 μl on agarose gel electrophoresis.
The ladder is composed of seven individual DNA fragments (in base pairs): 15000, 10000, 7500, 5000,
2500, 1000, and 250. It contains one intense band (2500bp) for easy orientation. To load 6 μl of the productwill provide 50 ng per DNA fragment with one intense fragment of 100 ng.

Buffer Contents
10 mM Tris-HCl (pH 8.4)
10 mM EDTA
0.02% Bromophenol Blue
5% Glycerol

Protocol

1. Directly load 3~6 μl of this product on the agarose gel
   (load 1 μl per 1 mm gel lane. The loading volume can be increased appropriately for wide lanes).
2. Electrophoresis condition: 0.7~1.0% agarose gel, 4~10v/cm voltage.
3. DNA marker can be visualized using ethidium bromide under UV light.

Recommendations

1. Directly use, do not heat before loading.
2. Please use new agarose gel and change electrophoresis
   buffer in time, so as not to affect the electrophoresis results.

MD110-D15000

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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