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Higher specificity and sensitivity

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• StorageStore in dark at -20°C
• DescriptionThe kit adopts the principle of SYBR^® Green I chimeric fluorescence to carry out miRNA fluorescence quantitative detection. The kit contains all reagents for quantitative miRNA fluorescence detection, including 2×miRcute Plus miRNA Premix, 50×ROX Reference Dye and Reverse Primer. Among them, 2× miRcute Plus miRNA Premix (With SYBR & ROX) is a new generation of premixed fluorescent quantitative PCR detection reagent specially developed for miRNA quantitative detection. The DNA Polymerase uses chemically modified hot-start polymerase, cooperating with a special buffer system, it has better reaction specificity, higher sensitivity, and accurate quantification can be carried out in a wider range.
• Features■ High sensitivity: Target miRNA of total RNA of pg grade can be detected.■ Strong specificity: The completely upgraded system can better distinguish single base differences and perfectly distinguish different miRNA within family.■ Good versatility: Suitable for various types of real time PCR instruments.
• Applications■ Detect reverse transcribed cDNA of miRNA by real time PCR
• Important Notes■ This kit must be used together with miRcute Plus miRNA First-Strand cDNA Kit (4992786/4992909).

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• CAPN2 correlates with insulin resistance states in PCOS as evidenced by multi-dataset analysisJournal of Ovarian Research2024-04-12 Read Article.. for mRNA/lncRNA analysis; and the miRcute Plus miRNA qPCR Kit (SYBR Green; FP411; TIANGEN; Beijing; China) for miRNA analysis.
• Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5pBMC Nephrology2024-04-12 Read Article.. TIANGEN Biotech; China) or the miRcute Plus miRNA qPCR Kit (SYBR Green) (cat: KR411; TIANGEN Biotech; China) with primers using cDNA
• Hypoxia treatment and resistance training alters microRNA profiling in rats skeletal muscleScientific Reports2024-04-10 Read ArticleThe miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen; China) was used for detection.
• MicroRNA Profiling Revealed the Mechanism of Enhanced Cold Resistance by Grafting in Melon ( Cucumis melo L.)Plants2024-04-02 Read Article.. China) and the expression levels were detected by the miRcute Plus miRNA qPCR kit (TIANGEN; Beijing; China).
• MiR-23b and miR-133 Cotarget TGF??2/NOTCH1 in Sheep Dermal Fibroblasts, Affecting Hair Follicle DevelopmentCells2024-03-21 Read Article.. ?? Green (Takara; Kusatsu; Japan) and the miRcute Plus miRNA qPCR Kit (SYBR Green) (TIANGEN; Beijing; China).

miRcute Plus miRNA qPCR Kit(SYBR Green)

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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