MP204-Pre-stained Protein Marker III (18~94 kDa)

Pre-stained Protein Marker
III (18~94 kDa)
Cat. no. 4992953
Storage: -30~-15°C for 1 year.
Concentration: 0.2~0.3 μg /μl of each protein.

Description
TIANGEN Pre-stained Protein Marker III is a protein molecular weight standard mixed with 5 pre-stained
proteins covering a range from 18 kDa to 94 kDa, which is 94 kDa, 60 kDa, 45 kDa, 27 kDa, and 18 kDa respectively. TIANGEN Pre-stained Protein Marker III is designed for
monitoring protein separation during SDS-polyacrylamide
gel electrophoresis and verification of Western Blot transfer efficiency.

Storage buffer
30 mM Tris-HCl (pH 7.5), 10 mM EDTA, 33% glycerol, 2% SDS.
1× SDS-PAGE buffer: 3.0 g Tris base (25 mM), 18.8 g Glycine (250 mM), 1 g SDS, dilute with ddH2O to 1 L.
1× Transfer buffer(Dry transfer): 5.8 g Tris base (48 mM),
2.9 g Glycine (39 mM) , 0.37 g SDS, 20% methanol, dilute with ddH2O to 1 L.

Protocol

  1. Thaw the marker at room temperature or 37~40°C for
    a few minutes to dissolve precipitated solids. Do not boil.
  2. Mix gently but thoroughly, to ensure the solution is homogeneous.
  3. Load 10 μl of the marker per well for gels with a
    thickness of 1.0 mm. The loading volume should be
    increased for thicker gels.

Electrophoresis
12~15% SDS-PAGE, 120~200 V for 30~50 min (Mini
Electrophoresis System)

Note

  1. Excessive electrophoresis run time may cause the diffusion of the protein bands.
  2. Pre-stained Protein Marker III is not suited for longtime (overnight) Western transfer in low voltage.

MP204-Pre-stained Protein Marker III (18~94 kDa)

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