OSR-M102-TGuide Blood Genomic DNA Kit

TGuide Blood Genomic DNA Kit

TGuide Blood Genomic DNA Kit
Cat. No. OSR-M102

Storage Conditions:
It can be stored dry at room temperature (15-30°C) for 12 months.
Other Related Reagents:
Buffer CL, Buffer GS

Product Description:
TGuide Blood Genomic DNA Kit is specially designed to cooperate with
TGuide M16 Nucleic Acid Extractor to extract DNA (including genomic DNA,
mitochondrial DNA and viral DNA) from whole blood, serum, plasma and
white blood cells. Reagents needed for cell lysis and protein degradation,
magnetic beads specifically adsorbing DNA, washing buffer and the like are
prepacked in the reagent cartridges, and purified DNA is eluted in a lowsalt buffer solution. The length of genomic DNA extracted by the kit is 20-30 kb, suitable for PCR or other enzymatic reactions.

Product Features:
Simple and fast: It takes 44/57 minutes to obtain ultrapure genomic DNA
from 200 µl/400 µl whole blood.
Reliable results: The obtained DNA is free from protein and RNA
contamination and is able to use for PCR or fluorescence quantitative PCR.
Safe and harmless: The kit and the operation process do not need to use
organic solvents harmful to human body such as phenol and chloroform.

Note: Read this note before using this kit.

1. This kit must be combined with TGuide M16 Automatic Nucleic Acid
   Extractor.
2. Repeated freezing and thawing of the sample should be avoided,
   otherwise the extraction yield will be decreased.

Operation steps:
For small amount of whole blood sample (≤ 400 µl), follow the whole
blood extraction procedure; For large and medium-amount whole blood
samples (500 µl-1.5 mL), follow the one-step lysis method.
Whole blood extraction procedure:

1. Add 200 µl/400 µl mammalian whole blood sample to the sample tube,
   and add 10 µl/20 µl Protease K to mix well.
   Note: Please add 200 µl Buffer GA for subsequent extraction when
   the sample volume is less than 200 µl. For anticoagulant blood of
   poultry, birds, amphibians or lower organisms, the red blood cells are
   nucleated cells. Therefore, the treated amount is 5-20 μl, top up with
   Buffer to a total 200 µl for subsequent extraction.
2. Place the sample tube in the well labeled “4” of the T-rack. Run the
   program No.102 (whole blood genomic DNA extraction program) and
   select the corresponding sample volume and final elution volume.

One-step lysis method procedure:

1. Add 500 μl-1.5 ml mammalian whole blood sample to the 1.5 ml/15 ml
   centrifuge tube.
   Note: For large volume samples (>600 μl, ≤1.5 ml), please replace it
   with a 15 ml sharp-bottom centrifuge tube for subsequent operation.
2. Add 2.5 times of Buffer CL (self-prepared) and mix up and down for 10
   times.
   Note: To facilitate the use of 1.5 ml centrifuge tubes for 500 μl-600 μl
   samples, Buffer CL with the same volume as mammalian whole blood
   samples can be added, and repeat the lysis step.
3. Centrifuge for 4 min at 2000×g and discard the supernatant.
   Note: If 1.5ml centrifuge tube is used, centrifuge at 12,000 rpm
   (~13,400 x g) for 1 min, and discard the supernatant.
4. Add 400 μl of Buffer GS (self-prepared) and 40 μl of Protease K to
   resuspend the precipitate.
5. Transfer the above mixed solution to a 1.5 ml sample tube.
6. Place the sample tube in the well 4 of the T-rack. Run the procedure
   No.102 (whole blood genomic DNA extraction procedure), and select
   the sample volume of 400 μl and the final elution volume.

OSR-M102-TGuide Blood Genomic DNA Kit

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This product is for scientific research use only. Do not use in medicine, clinical treatment, food or cosmetics.

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